In Vitro Lung Models and Their Application to Study SARS-CoV-2 Pathogenesis and Disease

被引：28

作者：

Heinen, Natalie ^{[1
]}

Kloehn, Mara ^{[1
]}

Steinmann, Eike ^{[1
]}

Pfaender, Stephanie ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Dept Mol & Med Virol, D-44801 Bochum, Germany

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 05期

关键词：

SARS-CoV-2; in vitro lung model; cell culture; human airway epithelial cell culture; human lung organoids; lung-on-a-chip; ex vivo lung; air-liquid interface; RESPIRATORY-SYNDROME-CORONAVIRUS; AIRWAY EPITHELIAL-CELLS; EX-VIVO; VIRUS-INFECTION; CILIATED CELLS; SARS; REPLICATION; RECEPTOR; CULTURE; TARGET;

D O I：

10.3390/v13050792

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

SARS-CoV-2 has spread across the globe with an astonishing velocity and lethality that has put scientist and pharmaceutical companies worldwide on the spot to develop novel treatment options and reliable vaccination for billions of people. To combat its associated disease COVID-19 and potentially newly emerging coronaviruses, numerous pre-clinical cell culture techniques have progressively been used, which allow the study of SARS-CoV-2 pathogenesis, basic replication mechanisms, and drug efficiency in the most authentic context. Hence, this review was designed to summarize and discuss currently used in vitro and ex vivo cell culture systems and will illustrate how these systems will help us to face the challenges imposed by the current SARS-CoV-2 pandemic.

引用

页数：17

共 121 条

[41] Regulation of immune responses by the airway epithelial cell landscape [J].

Hewitt, Richard J. ;

Lloyd, Clare M. .

NATURE REVIEWS IMMUNOLOGY, 2021, 21 (06) :347-362

[42] Human lung ex vivo infection models [J].

Hocke, Andreas C. ;

Suttorp, Norbert ;

Hippenstiel, Stefan .

CELL AND TISSUE RESEARCH, 2017, 367 (03) :511-524

[43] SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor [J].

Hoffmann, Markus ;

Kleine-Weber, Hannah ;

Schroeder, Simon ;

Krueger, Nadine ;

Herrler, Tanja ;

Erichsen, Sandra ;

Schiergens, Tobias S. ;

Herrler, Georg ;

Wu, Nai-Huei ;

Nitsche, Andreas ;

Mueller, Marcel A. ;

Drosten, Christian ;

Poehlmann, Stefan .

CELL, 2020, 181 (02) :271-+

[44] Flow-enhanced vascularization and maturation of kidney organoids in vitro [J].

Homan, Kimberly A. ;

Gupta, Navin ;

Kroll, Katharina T. ;

Kolesky, David B. ;

Skylar-Scott, Mark ;

Miyoshi, Tomoya ;

Mau, Donald ;

Valerius, M. Todd ;

Ferrante, Thomas ;

Bonventre, Joseph V. ;

Lewis, Jennifer A. ;

Morizane, Ryuji .

NATURE METHODS, 2019, 16 (03) :255-+

[45] SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo [J].

Hou, Yixuan J. ;

Chiba, Shiho ;

Halfmann, Peter ;

Ehre, Camille ;

Kuroda, Makoto ;

Dinnon, Kenneth H. ;

Leist, Sarah R. ;

Schafer, Alexandra ;

Nakajima, Noriko ;

Takahashi, Kenta ;

Lee, Rhianna E. ;

Mascenik, Teresa M. ;

Graham, Rachel ;

Edwards, Caitlin E. ;

Tse, Longping, V ;

Okuda, Kenichi ;

Markmann, Alena J. ;

Bartelt, Luther ;

de Silva, Aravinda ;

Margolis, David M. ;

Boucher, Richard C. ;

Randell, Scott H. ;

Suzuki, Tadaki ;

Gralinski, Lisa E. ;

Kawaoka, Yoshihiro ;

Baric, Ralph S. .

SCIENCE, 2020, 370 (6523) :1464-1468

[46] Lung Structure and the Intrinsic Challenges of Gas Exchange [J].

Hsia, Connie C. W. ;

Hyde, Dallas M. ;

Weibel, Ewald R. .

COMPREHENSIVE PHYSIOLOGY, 2016, 6 (02) :827-895

[47] Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China [J].

Huang, Chaolin ;

Wang, Yeming ;

Li, Xingwang ;

Ren, Lili ;

Zhao, Jianping ;

Hu, Yi ;

Zhang, Li ;

Fan, Guohui ;

Xu, Jiuyang ;

Gu, Xiaoying ;

Cheng, Zhenshun ;

Yu, Ting ;

Xia, Jiaan ;

Wei, Yuan ;

Wu, Wenjuan ;

Xie, Xuelei ;

Yin, Wen ;

Li, Hui ;

Liu, Min ;

Xiao, Yan ;

Gao, Hong ;

Guo, Li ;

Xie, Jungang ;

Wang, Guangfa ;

Jiang, Rongmeng ;

Gao, Zhancheng ;

Jin, Qi ;

Wang, Jianwei ;

Cao, Bin .

LANCET, 2020, 395 (10223) :497-506

[48] Animal and translational models of SARS-CoV-2 infection and COVID-19 [J].

Johansen, M. D. ;

Irving, A. ;

Montagutelli, X. ;

Tate, M. D. ;

Rudloff, I ;

Nold, M. F. ;

Hansbro, N. G. ;

Kim, R. Y. ;

Donovan, C. ;

Liu, G. ;

Faiz, A. ;

Short, K. R. ;

Lyons, J. G. ;

McCaughan, G. W. ;

Gorrell, M. D. ;

Cole, A. ;

Moreno, C. ;

Couteur, D. ;

Hesselson, D. ;

Triccas, J. ;

Neely, G. G. ;

Gamble, J. R. ;

Simpson, S. J. ;

Saunders, B. M. ;

Oliver, B. G. ;

Britton, W. J. ;

Wark, P. A. ;

Nold-Petry, C. A. ;

Hansbro, P. M. .

MUCOSAL IMMUNOLOGY, 2020, 13 (06) :877-891

[49] Coronaviruses and the human airway: a universal system for virus-host interaction studies [J].

Jonsdottir, Hulda R. ;

Dijkman, Ronald .

VIROLOGY JOURNAL, 2016, 13

[50] Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction [J].

Katsura, Hiroaki ;

Sontake, Vishwaraj ;

Tata, Aleksandra ;

Kobayashi, Yoshihiko ;

Edwards, Caitlin E. ;

Heaton, Brook E. ;

Konkimalla, Arvind ;

Asakura, Takanori ;

Mikami, Yu ;

Fritch, Ethan J. ;

Lee, Patty J. ;

Heaton, Nicholas S. ;

Boucher, Richard C. ;

Randell, Scott H. ;

Baric, Ralph S. ;

Tata, Purushothama Rao .

CELL STEM CELL, 2020, 27 (06) :890-+

← 1 2 3 4 5 6 7 8 9 10 →