A rapid and simplified extraction method of erythromycin from fermentation broth with bond elut C18 cartridge for analysis by HPLC

被引：8

作者：

Heydarian, SM ^{[1
]}

Ison, AP ^{[1
]}

Mirjalili, N ^{[1
]}

机构：

[1] Univ London Univ Coll, Dept Chem & Biochem Engn, Adv Ctr Biochem Engn, London WC1E 7JE, England

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 02期

关键词：

D O I：

10.1023/A:1008848719382

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Solid phase extraction of erythromycin from fermentation broth is described in this paper. This method results in 85 percent recovery of erythromycin A with a batch to batch variability of less than 3 percent. Each column can be used twice with 2-3% loss of recovery. The columns are regenerated immediately after each extraction. In comparison with liquid liquid extraction, solid phase extracts are cleaner, the method is less time consuming and exposure to hazardous solvents is reduced.

引用

页码：155 / 158

页数：4