Determination of α-tocopherolquinone in human serum samples by liquid chromatography with fluorescence detection and on-line post-column derivatization

被引：14

作者：

Pollok, D ^{[1
]}

Melchert, HU ^{[1
]}

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1056卷 / 1-2期

关键词：

derivatization; LC; zinc-reduction column; photoreactor; vitamin E quinones; tocopherolquinones;

D O I：

10.1016/j.chroma.2004.06.108

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC/fluorescence method with on-line post-column derivatization by a photoreactor was developed, where alpha-, beta + gamma- and delta-tocopherolquinone (TQ) are separated on a 250 mm x 4.6 mm RP-18 column. The LOD is about 250 pg for all TQs. In combination with a two-step sample preparation procedure, this method was successfully employed for measurement of alpha-TQ in human serum samples. Recovery for a-TQ from spiked serum was excellent (99 +/- 5%) and results of alpha-TQ determinations in 111 serum samples are reported. Additionally, possibilities for determination of other TQs in serum and alternative derivatization with a zinc reduction column are discussed. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：257 / 262

页数：6

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