HPLC study of chlorin e6 and its molecular complex with polyvinyl pyrrolidone

被引：16
作者：
Isakau, H. A.
Trukhacheva, T. V.
Zhebentyaev, A. I.
Petrov, P. T.
机构：
[1] RUE Belmedpreparaty, Sci Pharmaceut Ctr, Minsk 220007, BELARUS
[2] Vitebsk State Med Univ, Dept Pharm, Vitebsk 210602, BELARUS
来源：
BIOMEDICAL CHROMATOGRAPHY | 2007年 / 21卷 / 03期
关键词：
photolon; chlorin e6; HPLC; PVP;
D O I：
10.1002/bmc.762
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Several HPLC methods with UV detection were developed for qualitative and quantitative analysis of chlorin e, and photosensitizer Photolon either in the free form or upon pre-derivatization (methylation) under reversed- and normal-phase conditions. Optimum analysis conditions providing the best resolution of analytes were found at acidic pH where polar groups are completely protonated. The separation was performed by gradient elution with mobile phases of 0.08% trifluoroacetic acid and acetonitrile on an XTerra RP18 column. The method was specific, accurate and precise, allowing the analysis of chlorin e(6) in the presence of numerous degradation products useful in the manufacturing process and quality control of chlorin e(6) and Photolon. Copyright (c) 2007 John Wiley & Sons, Ltd.
引用
收藏
页码：318 / 325
页数：8
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