Identification of the Three Genes Involved in Controlling Production of a Phytotoxin Tropolone in Burkholderia plantarii

被引：12

作者：

Miwa, Shunpei ^{[1
]}

Kihira, Eri ^{[1
]}

Yoshioka, Akinori ^{[1
]}

Nakasone, Kaoru ^{[2
]}

Okamoto, Sho ^{[1
,4
]}

Hatano, Masaki ^{[3
]}

Igarashi, Masayuki ^{[3
]}

Eguchi, Yoko ^{[1
,5
]}

Kato, Akinori ^{[1
]}

Ichikawa, Natsuko ^{[6
]}

Sekine, Mitsuo ^{[6
]}

Fujita, Nobuyuki ^{[6
]}

Kanesaki, Yu ^{[7
]}

Yoshikawa, Hirofumi ^{[7
,8
]}

Utsumi, Ryutaro ^{[1
]}

机构：

[1] Kinki Univ, Grad Sch Agr, Dept Biosci, Nara, Japan

[2] Kinki Univ, Fac Engn, Dept Biotechnol & Chem, Hiroshima, Japan

[3] Inst Microbial Chem BIKAKEN, Tokyo, Japan

[4] Natl Inst Genet, Genet Strains Res Ctr, Shizuoka, Japan

[5] Kinki Univ, Fac Biol Oriented Sci & Technol, Dept Sci & Technol Food Safety, Wakayama, Japan

[6] Natl Inst Technol & Evaluat, Biol Resource Ctr, Tokyo, Japan

[7] Tokyo Univ Agr, NODAI Genome Res Ctr, Tokyo, Japan

[8] Tokyo Univ Agr, Dept Biosci, Tokyo, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2016年 / 198卷 / 11期

基金：

日本学术振兴会;

关键词：

TROPODITHIETIC ACID BIOSYNTHESIS; RICE SEEDLING BLIGHT; ESCHERICHIA-COLI; STIPITATIC ACID; PSEUDOMONAS; EXPRESSION; PROVIDES; PLASMID; SYSTEM;

D O I：

10.1128/JB.01028-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tropolone, a phytotoxin produced by Burkholderia plantarii, causes rice seedling blight. To identify genes involved in tropolone synthesis, we systematically constructed mutations in the genes encoding 55 histidine kinases and 72 response regulators. From the resulting defective strains, we isolated three mutants, KE1, KE2, and KE3, in which tropolone production was repressed. The deleted genes of these mutants were named troR1, troK, and troR2, respectively. The mutant strains did not cause rice seedling blight, and complementation experiments indicated that TroR1, TroK, and TroR2 were involved in the synthesis of tropolone in B. plantarii. However, tropolone synthesis was repressed in the TroR1 D52A, TroK H253A, and TroR2 D46A site-directed mutants. These results suggest that the putative sensor kinase (TroK) and two response regulators (TroR1 and TroR2) control the production of tropolone in B. plantarii. IMPORTANCE A two-component system is normally composed of a sensor histidine kinase (HK) and a cognate response regulator (RR) pair. In this study, HK (TroK) and two RRs (TroR1 and TroR2) were found to be involved in controlling tropolone production in B. plantarii. These three genes may be part of a bacterial signal transduction network. Such networks are thought to exist in other bacteria to regulate phytotoxin production, as well as environmental adaptation and signal transduction.

引用

页码：1604 / 1609

页数：6

共 21 条

[1] PSEUDOMONAS-PLANTARII SP-NOV, THE CAUSAL AGENT OF RICE SEEDLING BLIGHT [J].