Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data

被引：19

作者：

Ashdown, George W. ^{[1
,2
]}

Cope, Andrew ^{[3
]}

Wiseman, Paul W. ^{[4
,5
]}

Owen, Dylan M. ^{[1
,2
]}

机构：

[1] Kings Coll London, Dept Phys, London, England

[2] Kings Coll London, Randall Div Cell & Mol Biophys, London, England

[3] Kings Coll London, Acad Dept Rheumatol, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London, England

[4] McGill Univ, Dept Chem, Montreal, PQ, Canada

[5] McGill Univ, Dept Phys, Montreal, PQ, Canada

来源：

BIOPHYSICAL JOURNAL | 2014年 / 107卷 / 09期

基金：

欧洲研究理事会;

关键词：

ACTIN POLYMERIZATION; RETROGRADE FLOW; T-CELLS;

D O I：

10.1016/j.bpj.2014.09.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.

引用

页码：L21 / L23

页数：3

共 14 条

[1] F-actin polymerization and retrograde flow drive sustained PLCγ1 signaling during T cell activation [J].

Babich, Alexander ;

Li, Shuixing ;

O'Connor, Roddy S. ;

Milone, Michael C. ;

Freedman, Bruce D. ;

Burkhardt, Janis K. .

JOURNAL OF CELL BIOLOGY, 2012, 197 (06) :775-787

[2] Probing the integrin-actin linkage using high-resolution protein velocity mapping [J].

Brown, Claire M. ;

Hebert, Benedict ;

Kolin, David L. ;

Zareno, Jessica ;

Whitmore, Leanna ;

Horwitz, Alan Rick ;

Wiseman, Paul W. .

JOURNAL OF CELL SCIENCE, 2006, 119 (24) :5204-5214

[3] Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling [J].

Campi, G ;

Varma, R ;

Dustin, ML .

JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 202 (08) :1031-1036

[4] The many faces of actin: Matching assembly factors with cellular structures [J].

Chhabra, Ekta Seth ;

Higgs, Henry N. .

NATURE CELL BIOLOGY, 2007, 9 (10) :1110-1121

[5] FLEXURAL RIGIDITY OF MICROTUBULES AND ACTIN-FILAMENTS MEASURED FROM THERMAL FLUCTUATIONS IN SHAPE [J].

GITTES, F ;

MICKEY, B ;

NETTLETON, J ;

HOWARD, J .

JOURNAL OF CELL BIOLOGY, 1993, 120 (04) :923-934

[6] Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy [J].

Gustafsson, MGL .

JOURNAL OF MICROSCOPY, 2000, 198 (02) :82-87

[7] Spatiotemporal image correlation Spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells [J].

Hebert, B ;

Costantino, S ;

Wiseman, PW .

BIOPHYSICAL JOURNAL, 2005, 88 (05) :3601-3614

[8] Membrane mechanisms for signal transduction: The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes [J].

Kusumi, Akihiro ;

Fujiwara, Takahiro K. ;

Morone, Nobuhiro ;

Yoshida, Kenta J. ;

Chadda, Rahul ;

Xie, Min ;

Kasai, Rinshi S. ;

Suzuki, Kenichi G. N. .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2012, 23 (02) :126-144

[9] TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation [J].

Lillemeier, Bjoern F. ;

Moertelmaier, Manuel A. ;

Forstner, Martin B. ;

Huppa, Johannes B. ;

Groves, Jay T. ;

Davis, Mark M. .

NATURE IMMUNOLOGY, 2010, 11 (01) :90-U106

[10] Cell motility driven by actin polymerization [J].

Mogilner, A ;

Oster, G .

BIOPHYSICAL JOURNAL, 1996, 71 (06) :3030-3045

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