A novel methodology for imaging new bone formation around bioceramic bone substitutes

被引：8
作者：
Gorustovich, Alejandro A.
Sivak, Matias G.
Guglielmotti, Maria B.
机构：
[1] Natl Atom Energy Commiss, CNEA Reg Noroeste, Res Lab, Salta, Argentina
[2] Consejo Nacl Invest Cient & Tecn, Natl Res Council, RA-1033 Buenos Aires, DF, Argentina
[3] Univ Buenos Aires, Sch Dent, Dept Oral Pathol, Buenos Aires, DF, Argentina
来源：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2007年 / 81A卷 / 02期
关键词：
confocal laser scanning microscopy; bone; bioceramics;
D O I：
10.1002/jbm.a.31071
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In this study, Frost's bulk-staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码：443 / 445
页数：3
相关论文
共 13 条
[1]   Intracortical remodeling in adult rat long bones after fatigue loading [J].
Bentolila, V ;
Boyce, TM ;
Fyhrie, DP ;
Drumb, R ;
Skerry, TM ;
Schaffler, MB .
BONE, 1998, 23 (03) :275-281
[2]   Evaluation of an injectable bone substitute (βTCP/hydroxyapatite/hydroxy-propyl-methyl-cellulose) in severely osteopenic and aged rats [J].
Blouin, S. ;
Moreau, M. F. ;
Weiss, P. ;
Daculsi, G. ;
Basle, M. F. ;
Chappard, D. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2006, 78A (03) :570-580
[3]   VALIDITY OF THE BULK-STAINING TECHNIQUE TO SEPARATE ARTIFACTUAL FROM INVIVO BONE MICRODAMAGE [J].
BURR, DB ;
STAFFORD, T .
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 1990, (260) :305-308
[4]  
Frost H., 1960, HENRY FORD HOSP MEDI, V8, P25
[5]   In vivo bone regeneration with injectable calcium phosphate biomaterial:: A three-dimensional micro-computed tomographic, biomechanical and SEM study [J].
Gauthier, O ;
Müller, R ;
von Stechow, D ;
Lamy, B ;
Weiss, P ;
Bouler, JM ;
Aguado, E ;
Daculsi, G .
BIOMATERIALS, 2005, 26 (27) :5444-5453
[6]   Noninvasive bone replacement with a new injectable calcium phosphate biomaterial [J].
Gauthier, O ;
Khairoun, I ;
Bosco, J ;
Obadia, L ;
Bourges, X ;
Rau, C ;
Magne, D ;
Bouler, JM ;
Aguado, E ;
Daculsi, G ;
Weiss, P .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 66A (01) :47-54
[7]   Application of histomorphometric methods to the study of bone repair [J].
Gerstenfeld, LC ;
Wronski, TJ ;
Hollinger, JO ;
Einhorn, TA .
JOURNAL OF BONE AND MINERAL RESEARCH, 2005, 20 (10) :1715-1722
[8]   Increased osteogenesis elicited by boron-modified bioactive glass particles in the SiO2-CaO-P2O5-Na2O system:: A histomorphometric study in rats [J].
Gorustovich, A ;
Guglielmotti, MB ;
López, JMP ;
Cabrini, RL .
BIOCERAMICS 17, 2005, 284-286 :913-916
[9]  
Gorustovich A, 2002, INT J ORAL MAX IMPL, V17, P644
[10]   Biological performance of boron-modified bioactive glass particles implanted in rat tibia bone marrow [J].
Gorustovich, Alejandro A. ;
Lopez, Jose M. Porto ;
Guglielmotti, MAria B. ;
Cabrini, Romulo L. .
BIOMEDICAL MATERIALS, 2006, 1 (03) :100-105
12