Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing

被引：69
作者：
Beil, B
Screaton, G
Stamm, S
机构：
[1] MAX PLANCK INST PSYCHIAT,D-82152 PLANEGG,GERMANY
[2] JOHN RADCLIFFE HOSP,INST MOL MED,OXFORD OX3 9DU,ENGLAND
来源：
DNA AND CELL BIOLOGY | 1997年 / 16卷 / 06期
基金：
英国惠康基金;
关键词：
D O I：
10.1089/dna.1997.16.679
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A yeast two-hybrid screen was performed to find new factors involved in pre-mRNA splicing. Using SC35 as a bait, we isolated a human cDNA bearing high homology to the Drosophila transformer-2 (TRA-2) protein, This cDNA was named htra2-beta1, htra2-beta1 is a nuclear protein that colocalizes with SC35 in a speckled pattern, It interacts with several SR proteins tested in yeast. A second form named htra2-beta2 is generated by alternative splicing, This isoform gives rise to a truncated protein without an SR domain, Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these two isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in regulation of htra2-beta activity.
引用
收藏
页码：679 / 690
页数：12
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