Species identification of clinically important Aeromonas spp. by restriction fragment length polymorphism of 16S rDNA

The aim of the study was to characterize 16S rDNA of Aeromonas spp. to rapidly identify clinically important species of these bacteria. Sequence analysis of published 16S rDNA for unique restriction sites revealed prospect of species identification. Extraction of genomic DNA followed by amplification and step-by-step restriction endonuclease digestion of 16S rDNA was able to identify Aeromonas spp. of medical significance. Validation of the method was performed by subjecting 53 Aeromonas strains of multiple origin to similar treatment. Results of the study were in agreement with corresponding species of the isolates. The method developed offers an easily interpretable tool for the identification of Aeromonas spp. of clinical relevance. The developed methodology should facilitate routine laboratory diagnosis of Aeromonas spp. from clinical cases to species level.