On-chip multi-gas incubation for microfluidic cell cultures under hypoxia

被引：14

作者：

Takano, Atsushi ^{[1
]}

Tanaka, Masato ^{[1
]}

Futai, Nobuyuki ^{[2
]}

机构：

[1] Tokyo Denki Univ, Dept Mat & Life Sci, Saitama 3500394, Japan

[2] Shibaura Inst Technol, Dept Mech Engn, Tokyo 1358548, Japan

来源：

BIOMICROFLUIDICS | 2014年 / 8卷 / 06期

关键词：

OXYGEN;

D O I：

10.1063/1.4902941

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a simple system that regulates CO2 and O-2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO(2) and desired pO(2) levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth. (C) 2014 AIP Publishing LLC.

引用

页数：4

共 13 条

[1] Limitations to oxygen diffusion and equilibration in in vitro cell exposure systems in hyperoxia and hypoxia [J].

Allen, CB ;

Schneider, BK ;

White, CW .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2001, 281 (04) :L1021-L1027

[2] IN THE ABSENCE OF CATALYTIC METALS ASCORBATE DOES NOT AUTOXIDIZE AT PH-7 - ASCORBATE AS A TEST FOR CATALYTIC METALS [J].

BUETTNER, GR .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1988, 16 (01) :27-40

[3] Oxygen sensing and molecular adaptation to hypoxia [J].

Bunn, HF ;

Poyton, RO .

PHYSIOLOGICAL REVIEWS, 1996, 76 (03) :839-885

[4] Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia [J].

Carreau, Aude ;

El Hafny-Rahbi, Bouchra ;

Matejuk, Agata ;

Grillon, Catherine ;

Kieda, Claudine .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2011, 15 (06) :1239-1253

[5] A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment [J].

Funamoto, Kenichi ;

Zervantonakis, Ioannis K. ;

Liu, Yuchun ;

Ochs, Christopher J. ;

Kim, Choong ;

Kamm, Roger D. .

LAB ON A CHIP, 2012, 12 (22) :4855-4863

[6] Rapid prototyping of microstructures with bell-shaped cross-sections and its application to deformation-based microfluidic valves [J].

Futai, N ;

Gu, W ;

Takayama, S .

ADVANCED MATERIALS, 2004, 16 (15) :1320-+

[7] Hypoxia-induced apoptosis of dorsal root ganglion neurons is associated with DNA damage recognition and cell cycle disruption in rats [J].

Honma, H ;

Gross, L ;

Windebank, AJ .

NEUROSCIENCE LETTERS, 2004, 354 (02) :95-98

[8] CONCERTED PROTON-ELECTRON TRANSFER BETWEEN ASCORBIC-ACID AND CYTOCHROME-B561 [J].

NJUS, D ;

JALUKAR, V ;

ZU, J ;

KELLEY, PM .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1991, 54 (06) :S1179-S1183

[9] Regulation of angiogenesis by hypoxia: role of the HIF system [J].

Pugh, CW ;

Ratcliffe, PJ .

NATURE MEDICINE, 2003, 9 (06) :677-684

[10] The role of oxygen availability in embryonic development and stem cell function [J].

Simon, M. Celeste ;

Keith, Brian .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (04) :285-296

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