fbl Gene as a Species-Specific Target for Staphylococcus lugdunensis Identification

被引：9

作者：

Chatzigeorgiou, Kalliopi-Stavroula ^{[1
]}

Siafakas, Nikolaos ^{[1
]}

Petinaki, Efthymia ^{[2
]}

Zerva, Loukia ^{[1
]}

机构：

[1] ATTIKON Univ Hosp, Lab Clin Microbiol, Athens 12462, Greece

[2] Univ Thessaly, Sch Med, Dept Microbiol, Larisa, Greece

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2010年 / 24卷 / 02期

关键词：

fbl gene; identification; PCR; Staphylococcus lugdunensis; fbl protein; expression; POLYMERASE-CHAIN-REACTION; LEVEL IDENTIFICATION; TUF GENE; PCR; STRAINS; PROTEIN; RNA; DNA;

D O I：

10.1002/jcla.20352

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed as a suitable nucleic acid target The gene was detected in all 17 S lugdunensis isolates examined, while no amplification product was obtained from 98 isolates representing 11 staphylococcal and 17 nonstaphylococcal species Forty-seven percent of the S. lugdunensis strains produced a positive slide coagulase reaction, which is consistent with varying levels of Fbl protein expression within the species J. Clin Lab Anal 24:119-122, 2010. (C) 2010 Wiley-Liss, Inc

引用

页码：119 / 122

页数：4

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