Neisseria meningitidis porin PorB interacts with mitochondria and protects cells from apoptosis

被引：139

作者：

Massari, P ^{[1
]}

Ho, Y ^{[1
]}

Wetzler, LM ^{[1
]}

机构：

[1] Boston Univ, Sch Med, Evans Biomed Res Ctr, Boston Med Ctr, Boston, MA 02118 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 16期

关键词：

cell death; voltage-dependent anion channel (VDAC); membrane potential; staurosporine;

D O I：

10.1073/pnas.97.16.9070

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Neisserial porins are strong immune adjuvants and B cell activators. The effect of neisserial porin PorB on activation-induced cell death was investigated, as a potential additional mechanism of the porin's immunopotentiating ability. Neisserial porins interact with target cells to localize intracellularly in the mitochondrial compartment without negatively affecting cellular survival. Pretreatment with Neisseria meningitidis PorB porin decreased or abrogated the mitochondrial damage induced by apoptotic stimuli. In addition, end stage determinants of apoptosis, including DNA breakdown, were diminished by PorB. Immunoprecipitation experiments revealed that PorB interacts with the mitochondrial porin VDAC (voltage-dependent anion channel). The mechanism of the antiapoptotic effect of neisserial porins could be explained by the protein-protein interaction of PorB with VDAC, similar to the interaction of VDAC with antiapoptotic Bcl-2 proteins, resulting in an enhancement of cell survival and continued activation of B cells.

引用

页码：9070 / 9075

页数：6

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