Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

Back pain is a significant clinical concern often associated with degeneration of the intervertebral disc (IVD). Tissue engineering strategies may provide a viable IVD replacement therapy; however, an ideal biomaterial scaffold has yet to be identified. One candidate material is carboxymethylcellulose (CMC), a water-soluble derivative of cellulose. In this study, 90 and 250 kDa CMC polymers were modified with functional methacrylate groups and photocrosslinked to produce hydrogels at different macromer concentrations. At 7 days, bovine nucleus pulposus (NP) cells encapsulated in these hydrogels were viable, with values for the elastic modulus ranging from 1.07 +/- 0.06 to 4.29 +/- 1.25 kPa. Three specific formulations were chosen for further study based on cell viability and mechanical integrity assessments: 4% 90 kDa, 2% 250 kDa and 3% 250 kDa CIVIC. The equilibrium weight swelling ratio of these formulations remained steady throughout the 2 week study (46.45 +/- 3.14, 48.55 +/- 2.91 and 42.41 +/- 3.06, respectively). The equilibrium Young's modulus of all cell-laden and cell-free control samples decreased over time, with the exception of cell-laden 3% 250 kDa CIVIC constructs, indicating an interplay between limited hydrolysis of interchain crosslinks and the elaboration of a functional matrix. Histological analyses of 3% 250 kDa CMC hydrogels confirmed the presence of rounded cells in lacunae and the pericellular deposition of chondroitin sulfate proteoglycan, a phenotypic NP marker. Taken together, these studies support the use of photocrosslinked CMC hydrogels as tunable biomaterials for NP cell encapsulation. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.