Tetracycline-inducible shRNA targeting antisense long non-coding RNA HIF1A-AS2 represses the malignant phenotypes of bladder cancer

被引：87

作者：

Chen, Mingwei ^{[1
,2
,3
]}

Zhuang, Chengle ^{[1
,4
]}

Liu, Yuchen ^{[1
]}

Li, Jianfa ^{[1
,4
]}

Dai, Fen ^{[3
]}

Xia, Ming ^{[5
]}

Zhan, Yonghao ^{[1
,4
]}

Lin, Junhao ^{[1
,4
]}

Chen, Zhicong ^{[1
,4
]}

He, Anbang ^{[1
,3
]}

Xu, Wen ^{[1
]}

Zhao, Guoping ^{[1
,6
]}

Guo, Yinglu ^{[7
]}

Cai, Zhiming ^{[1
,3
,4
,7
]}

Huang, Weiren ^{[1
,3
,4
,7
]}

机构：

[1] Shenzhen Univ, Affiliated Hosp 1, Key Lab Med Reprogramming Technol, Clin Inst,Anhui Med Univ,Shenzhen Peoples Hosp 2, Shenzhen 518039, Guangdong, Peoples R China

[2] Zhejiang Univ, Affiliated Hosp 4, Dept Urol, Sch Med, Yiwu 322000, Zhejiang, Peoples R China

[3] Anhui Med Univ, Hefei 230032, Anhui, Peoples R China

[4] Shantou Univ, Coll Med, Shantou 515041, Guangdong, Peoples R China

[5] Southen Med Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510630, Guangdong, Peoples R China

[6] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 200000, Peoples R China

[7] Peking Univ, Hosp 1, Inst Urol, Dept Urol,Natl Urol Canc Ctr, Beijing 100034, Peoples R China

来源：

CANCER LETTERS | 2016年 / 376卷 / 01期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

HIF1A-AS2; Bladder cancer; LncRNAs; Tetracycline-inducible; Synthetic biology; CELL-GROWTH; TUMOR-GROWTH; INVASION; IDENTIFICATION; PROGRESSION; EXPRESSION; COMPLEX; BIOLOGY;

D O I：

10.1016/j.canlet.2016.03.037

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Various studies have indicated that long non-coding RNAs (lncRNAs) play vital roles in the cancer development and progression. LncRNA hypoxia inducible factor 1 alpha antisense RNA-2 (HIF1A-AS2) is upregulated in gastric carcinomas and knockdown of HIF1A-AS2 expression by siRNA could inhibit cell proliferation in vitro and tumorigenesis in vivo. Inspired by these observations, we hypothesized that HIF1A-AS2 possibly plays the analogous roles in bladder cancer. In our study, we first reported that HIF1A-AS2 was up-regulated in bladder cancer tissues and cells, and HIF1A-AS2 expression level in bladder cancer tissues is positively associated with advanced clinical pathologic grade and TNM phase. Cell proliferation inhibition, cell migration suppression and apoptosis induction were observed by silencing HIF1A-AS2 in bladder cancer T24 and 5637 cells. Overexpression of HIF1A-AS2 in SV-HUC-1 cells could promote cell proliferation, cell migration and anti-apoptosis. Besides, we utilized the emerging technology of medical synthetic biology to design tetracycline-inducible small hairpin RNA (shRNA) vector which specifically silenced HIFI A-AS2 in a dosage-dependent manner to inhibit the progression of human bladder cancer. In conclusion, our data suggested that HIF1A-AS2 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer. Synthetic "tetracycline-on" switch system that quantitatively controlled the expression of HIF1A-AS2 in bladder cancer can inhibit the progression of bladder cancer cells in a dosage-dependent manner. Our findings provide new insights into the role of the IncRNA HIF1A-AS2 in the bladder cancer. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

引用

页码：155 / 164

页数：10

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