Overexpression of MicA induces production of OmpC-enriched outer membrane vesicles that protect against Salmonella challenge

被引：29

作者：

Choi, Hyun-il ^{[1
]}

Kim, Moonjeong ^{[2
]}

Jeon, Jinseong ^{[1
]}

Han, Jin Kwan ^{[1
]}

Kim, Kwang-sun ^{[2
]}

机构：

[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Dept Life Sci, Pohang 37673, Gyeongsangbuk D, South Korea

[2] Pusan Natl Univ, Chem Inst Funct Mat, Dept Chem, Busan 46241, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 490卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Outer membrane vesicles; MicA; Salmonella; Immunity; Vaccine; BACTERIA-INDUCED LETHALITY; ESCHERICHIA-COLI; EXTRACELLULAR VESICLES; CELL RESPONSES; RNA; IMMUNITY; RELEASE; IDENTIFICATION; BIOGENESIS; INFECTION;

D O I：

10.1016/j.bbrc.2017.06.152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Outer membrane vesicles (OMVs) derived from bacteria are promising candidates for subunit vaccines. Stresses that modulate the composition of outer membrane proteins (OMPs) are important for OMV synthesis. Small RNAs (sRNAs) expressed in response to stress regulate OMPs, although the mechanism underlying sRNA-mediated OMV biogenesis and its utility for developing vaccine platforms remains to be elucidated. Here, we characterized the role of a sRNA, MicA, which regulates OmpA, a major OMP involved in both production of OMVs and reactive immunity against Salmonella challenge. A Salmonella strain overexpressing MicA generated more OMVs than a control strain. In addition, OmpC was the major component of MicA-derived OMV proteins. MicA-derived OMVs induced Thl-and Th17-type immune responses in vitro and reduced Salmonella-mediated lethality in a mouse model. Thus, OmpA-regulatory sRNA-derived OMVs may facilitate production of Salmonella-protective vaccines. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：991 / 996

页数：6

共 35 条

[1] Membrane vesicles are immunogenic facsimiles of Salmonella typhimurium that potently activate dendritic cells, prime B and T cell responses, and stimulate protective immunity in vivo [J].