Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells

被引：13

作者：

Suphaphiphat, Pirada ^{[1
]}

Keiner, Bjoern ^{[1
]}

Trusheim, Heidi ^{[1
]}

Crotta, Stefania ^{[1
]}

Tuccino, Annunziata Barbara ^{[1
]}

Zhang, Pu ^{[1
]}

Dormitzer, Philip R. ^{[1
]}

Mason, Peter W. ^{[1
]}

Franti, Michael ^{[1
]}

机构：

[1] Novartis Vaccines & Diagnost, Cambridge, MA 02139 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 07期

关键词：

SPECIES IDENTIFICATION; VACCINE PRODUCTION; RIBOSOMAL-RNA; GENERATION; SYSTEM; ESTABLISHMENT; TRANSCRIPTION; PROMOTER; DNA;

D O I：

10.1128/JVI.01925-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.

引用

页码：3721 / 3725

页数：5

共 17 条

[1] MOLECULAR MECHANISMS GOVERNING SPECIES-SPECIFIC TRANSCRIPTION OF RIBOSOMAL-RNA
BELL, SP
PIKAARD, CS
REEDER, RH
TJIAN, R
[J]. CELL, 1989, 59 (03) : 489 - 497
[2] ASSEMBLY OF ALTERNATIVE MULTIPROTEIN COMPLEXES DIRECTS RIBOSOMAL-RNA PROMOTER SELECTIVITY
BELL, SP
JANTZEN, HM
TJIAN, R
[J]. GENES & DEVELOPMENT, 1990, 4 (06) : 943 - 954
[3] Proteolytic activation of influenza viruses by serine proteases TMPRSS2 and HAT from human airway epithelium
Böttcher, Eva
Matrosovich, Tatyana
Beyerle, Michaela
Klenk, Hans-Dieter
Garten, Wolfgang
Matrosovich, Mikhail
[J]. JOURNAL OF VIROLOGY, 2006, 80 (19) : 9896 - 9898
[4] Species identification in cell culture: a two-pronged molecular approach
Cooper, Jason K.
Sykes, Greg
King, Steve
Cottrill, Karin
Ivanova, Natalia V.
Hanner, Robert
Ikonomi, Pranvera
[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2007, 43 (10) : 344 - 351
[5] Rescue of influenza A virus from recombinant DNA
Fodor, E
Devenish, L
Engelhardt, OG
Palese, P
Brownlee, GG
García-Sastre, A
[J]. JOURNAL OF VIROLOGY, 1999, 73 (11) : 9679 - 9682
[6] SPECIES-SPECIFICITY OF TRANSCRIPTION BY RNA-POLYMERASE-I
HEIX, J
GRUMMT, I
[J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 1995, 5 (05) : 652 - 656
[7] A DNA transfection system for generation of influenza A virus from eight plasmids
Hoffmann, E
Neumann, G
Kawaoka, Y
Hobom, G
Webster, RG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) : 6108 - 6113
[8] Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication
Lutz, A
Dyall, J
Olivo, PD
Pekosz, A
[J]. JOURNAL OF VIROLOGICAL METHODS, 2005, 126 (1-2) : 13 - 20
[9] Establishment of canine RNA polymerase I-driven reverse genetics for influenza a virus: Its application for H5N1 vaccine production
Murakami, Shin
Horimoto, Taisuke
Yamada, Shinya
Kakugawa, Satoshi
Goto, Hideo
Kawaoka, Yoshihiro
[J]. JOURNAL OF VIROLOGY, 2008, 82 (03) : 1605 - 1609
[10] Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies
Nakagawa, N
Maeda, A
Kase, T
Kubota, R
Okuno, Y
[J]. JOURNAL OF VIROLOGICAL METHODS, 1999, 79 (01) : 113 - 120

← 1 2 →