A rabbit model of non-typhoidal Salmonella bacteremia

被引：6
作者：
Panda, Aruna [1 ,2 ,3 ]
Tatarov, Ivan [1 ,2 ]
Masek, Billie Jo [8 ]
Hardick, Justin [9 ]
Crusan, Annabelle [1 ,2 ]
Wakefield, Teresa [10 ]
Carroll, Karen [10 ]
Yang, Samuel [8 ]
Hsieh, Yu-Hsiang [8 ]
Lipsky, Michael M. [1 ,2 ]
McLeod, Charles G. [1 ,2 ]
Levine, Myron M. [3 ,4 ,5 ,6 ,7 ]
Rothman, Richard E. [8 ,9 ]
Gaydos, Charlotte A. [8 ,9 ]
DeTolla, Louis J. [1 ,2 ,3 ,4 ]
机构：
[1] Univ Maryland, Sch Med, Program Comparat Med, Baltimore, MD 21201 USA
[2] Univ Maryland, Sch Med, Dept Pathol, Baltimore, MD 21201 USA
[3] Univ Maryland, Sch Med, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA
[4] Univ Maryland, Sch Med, Dept Med Infect Dis, Baltimore, MD 21201 USA
[5] Univ Maryland, Sch Med, Ctr Vaccine Dev, Div Infect Dis & Trop Pediat,Dept Pediat, Baltimore, MD 21201 USA
[6] Univ Maryland, Sch Med, Dept Microbiol, Baltimore, MD 21201 USA
[7] Univ Maryland, Sch Med, Dept Immunol, Baltimore, MD 21201 USA
[8] Johns Hopkins Univ, Dept Emergency Med, Baltimore, MD USA
[9] Johns Hopkins Univ, Dept Med, Baltimore, MD USA
[10] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
来源：
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2014年 / 37卷 / 04期
关键词：
Bacteremia; Non-typhoidal Salmonella infections; Rabbit model; TYPHI; TYPHIMURIUM; MECHANISMS; VIRULENCE; IMMUNITY; CHILDREN; SEPSIS; BLOOD;
D O I：
10.1016/j.cimid.2014.05.004
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Bacteremia is an important cause of morbidity and mortality in humans. In this study, we focused on the development of an animal model of bacteremia induced by non-typhoidal Salmonella. New Zealand White rabbits were inoculated with a human isolate of non-typhoidal Salmonella strain CVD J73 via the intra-peritoneal route. Blood samples were collected at specific time points and at euthanasia from infected rabbits. Additionally, tissue samples from the heart, lungs, spleen, gastrointestinal tract, liver and kidneys were obtained at euthanasia. All experimentally infected rabbits displayed clinical signs of disease (fever, dehydration, weight loss and lethargy). Tissues collected at necropsy from the animals exhibited histopathological changes indicative of bacteremia. Non-typhoidal Salmonella bacteria were detected in the blood and tissue samples of infected rabbits by microbiological culture and real-time PCR assays. The development of this animal model of bacteremia could prove to be a useful tool for studying how non-typhoidal Salmonella infections disseminate and spread in humans. (C) 2014 Elsevier Ltd. All rights reserved.
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页码：211 / 220
页数：10
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