Novel Approach to Prepare Ultrathin Lignocellulosic Film for Monitoring Enzymatic Hydrolysis Process by Quartz Crystal Microbalance

被引:21
作者
Jiang, Chong [1 ]
Cao, Tingyue [1 ]
Wu, Wenjuan [1 ]
Song, Junlong [1 ]
Jin, Yongcan [1 ]
机构
[1] Nanjing Forestry Univ, Wood Chem Lab, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat F, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China
来源
ACS SUSTAINABLE CHEMISTRY & ENGINEERING | 2017年 / 5卷 / 05期
基金
中国国家自然科学基金;
关键词
Ultrathin film; Lignocellulosic biomass; LiCl/DMSO solvent system; Quartz crystal microbalance (QCM); Cellulase adsorption; Enzymatic hydrolysis; CHEMICAL-COMPOSITION; SURFACE LIGNIN; THIN-FILMS; CELLULOSE; DISSOLUTION; CELLULASES; ADSORPTION; BIOCONVERSION; NANOFIBRILS; INHIBITION;
D O I
10.1021/acssuschemeng.6b02884
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The applications of quartz crystal microbalance (QCM) rely heavily on the preparation of ultrathin films. So far, techniques on direct lignocellulosic film making without components isolation have been hardly investigated. In this work, a novel approach was developed to prepare spin-coated ultrathin films based on the complete dissolution of ball-milled wood in LiCI/DMSO solvent system. The surface analysis and elemental composition of the films respectively using atomic force microscopy and X-ray photoelectron spectroscopy proved that an even-textured lignocellulosic film could be formed on QCM gold sensors. The prepared ultrathin films were successfully applied on monitoring the enzymatic hydrolysis process in situ and in real time by QCM. The changes of QCM frequency showed clearly that the enzymatic hydrolysis of lignocellulosic materials could be divided into three stages, including cellulase adsorption, fast substrate hydrolysis, and slow substrate hydrolysis. The adsorption and hydrolysis processes were fitted with Lagergren and Boltzmann-sigmoidal kinetic models, respectively, indicating that cellulase adsorption on lignin and cellulose is competitive and that lignin inhibits the enzymatic hydrolysis of cellulose.
引用
收藏
页码:3837 / 3844
页数:8
相关论文
共 45 条
  • [1] Influence of adsorbed polyelectrolytes on pore size distribution of a water-swollen biomaterial
    Aarne, Niko
    Kontturi, Eero
    Laine, Janne
    [J]. SOFT MATTER, 2012, 8 (17) : 4740 - 4749
  • [2] [Anonymous], 2010, CELLIC CTEC2 HTEC2 E
  • [3] Weak lignin-rinding enzymes - A novel approach to improve activity of cellulases for hydrolysis of lignocellulosics
    Berlin, A
    Gilkes, N
    Kurabi, A
    Bura, R
    Tu, MB
    Kilburn, D
    Saddler, J
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2005, 121 (1-3) : 163 - 170
  • [4] Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
    Berlin, Alex
    Balakshin, Mikhail
    Gilkes, Neil
    Kadla, John
    Maximenko, Vera
    Kubo, Satoshi
    Saddler, Jack
    [J]. JOURNAL OF BIOTECHNOLOGY, 2006, 125 (02) : 198 - 209
  • [5] XPS STUDIES OF THE OXYGEN-1S AND OXYGEN-2S LEVELS IN A WIDE-RANGE OF FUNCTIONAL POLYMERS
    BRIGGS, D
    BEAMSON, G
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (11) : 1517 - 1523
  • [6] ADHESION BETWEEN PLASMA-TREATED CELLULOSIC MATERIALS AND POLYETHYLENE
    CARLSSON, CMG
    STROM, G
    [J]. SURFACE AND INTERFACE ANALYSIS, 1991, 17 (07) : 511 - 515
  • [7] A comparison of pretreatment methods for bioethanol production from lignocellulosic materials
    Conde-Mejia, Carolina
    Jimenez-Gutierrez, Arturo
    El-Halwagi, Mahmoud
    [J]. PROCESS SAFETY AND ENVIRONMENTAL PROTECTION, 2012, 90 (03) : 189 - 202
  • [8] Dorris G.M., 1978, CELL CHEM TECHNOL, V12, P9
  • [9] BIOSYNTHESIS AND CONSTITUTION OF LIGNIN
    FREUDENBERG, K
    [J]. NATURE, 1959, 183 (4669) : 1152 - 1155
  • [10] MEASUREMENT OF CELLULASE ACTIVITIES
    GHOSE, TK
    [J]. PURE AND APPLIED CHEMISTRY, 1987, 59 (02) : 257 - 268