PGC-1β-Regulated mitochondrial biogenesis and function in myotubes is mediated by NRF-1 and ERRα

被引：135

作者：

Shao, Di ^{[2
,3
]}

Liu, Yang ^{[2
,3
]}

Liu, Xiaojun ^{[2
,3
]}

Zhu, Liuluan ^{[2
,3
]}

Cui, Ying ^{[2
,3
]}

Cui, Anfang ^{[2
,3
]}

Qiao, Aijun ^{[2
,3
]}

Kong, Xingxing ^{[2
,3
]}

Liu, Yong ^{[4
]}

Chen, Quan ^{[4
]}

Gupta, Nishith ^{[1
]}

Fang, Fude ^{[2
,3
]}

Chang, Yongsheng ^{[2
,3
]}

机构：

[1] Humboldt Univ, Dept Mol Parasitol, D-10115 Berlin, Germany

[2] Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China

[3] Peking Union Med Coll, Beijing 100005, Peoples R China

[4] Chinese Acad Sci, Inst Zool, Natl Key Lab Biomembrane & Membrane Biotechnol, Lab Apoptosis & Canc Biol, Beijing 100101, Peoples R China

来源：

MITOCHONDRION | 2010年 / 10卷 / 05期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

PGC-1; beta; NRF-1; ERR alpha; Cytochrome c; Mitochondrial biogenesis; Energy metabolism; HUMAN SKELETAL-MUSCLE; RECEPTOR-ALPHA; ADAPTIVE THERMOGENESIS; INSULIN-RESISTANCE; OXIDATIVE-PHOSPHORYLATION; INDUCIBLE COACTIVATOR; ENERGY-METABOLISM; PGC-1; FAMILY; EXPRESSION; TRANSCRIPTION;

D O I：

10.1016/j.mito.2010.05.012

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) coactivator-1 beta (PGC-1 beta) is a well-established regulator of the beta-oxidation of fatty acids and the oxidative phosphorylation in mitochondria. However, the underlying mechanism of PGC-1 beta action remains elusive. This study reveals that PGC-1 beta is highly induced during myogenic differentiation and knockdown of endogenous PGC-1 beta by siRNA leads to a decrease in the expression of several mitochondria-related genes. In consistence, the over-expression of PGC-1 beta stimulates its target genes such as cytochrome c, ATP synthase beta and ALAS-1 by its interaction with two transcriptional factors, NRF-1 and ERR alpha. The deletion or mutation of NRF-1 and/or ERR alpha binding sites in target gene promoters attenuates their activation by PGC-1 beta. Moreover, inhibition of NRF-1 or ERR alpha by siRNA ablated the aforesaid function of PGC-1 beta and compromised the oxidative phosphorylation and mitochondrial biogenesis. Taken together, these results confirm the direct interaction of NRF-1 and ERR alpha with PGC-1 beta, and their participation in mitochondrial biogenesis and respiration. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：516 / 527

页数：12

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