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Gene expression regulatory networks in Trypanosoma brucei: insights into the role of the mRNA-binding proteome
被引:81
作者:

Lueong, Smiths
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Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Merce, Clementine
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机构:
Univ Heidelberg ZMBH, Zentrum Mol Biol, DKFZ ZMBH Alliance, Neuenheimer Feld 282, Heidelberg 69120, Germany
Deutsch Krebsforschungszentrum DKFZ, Mol Genome Anal, Neuenheimer Feld 460, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Fischer, Bernd
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机构:
Deutsch Krebsforschungszentrum DKFZ, Computat Genome Biol, Neuenheimer Feld 580, Heidelberg 69120, Germany Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Hoheisel, Jorg D.
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h-index: 0
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Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Erben, Esteban D.
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机构:
Univ Heidelberg ZMBH, Zentrum Mol Biol, DKFZ ZMBH Alliance, Neuenheimer Feld 282, Heidelberg 69120, Germany Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany
机构:
[1] Deutsch Krebsforschungszentrum DKFZ, Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany
[2] Univ Heidelberg ZMBH, Zentrum Mol Biol, DKFZ ZMBH Alliance, Neuenheimer Feld 282, Heidelberg 69120, Germany
[3] Deutsch Krebsforschungszentrum DKFZ, Computat Genome Biol, Neuenheimer Feld 580, Heidelberg 69120, Germany
[4] Deutsch Krebsforschungszentrum DKFZ, Mol Genome Anal, Neuenheimer Feld 460, D-69120 Heidelberg, Germany
关键词:
BLOOD-STREAM;
RECOMBINATIONAL CLONING;
CCCH PROTEIN;
IDENTIFICATION;
COMPLEX;
YEAST;
DIFFERENTIATION;
REVEALS;
DEADENYLATION;
CYTOKINESIS;
D O I:
10.1111/mmi.13328
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequences of the protein-mRNA interactions. Here, we provide an overview of the proteins that bind to mRNAs and their functions in the pathogenic bloodstream form of Trypanosoma brucei. We describe the production of a small collection of open-reading frames encoding proteins potentially involved in mRNA metabolism. With this ORFeome collection, we used tethering to screen for proteins that play a role in post-transcriptional control. A yeast two-hybrid screen showed that several of the discovered repressors interact with components of the CAF1/NOT1 deadenylation complex. To identify the RNA-binding proteins, we obtained the mRNA-bound proteome. We identified 155 high-confidence candidates, including many not previously annotated as RNA-binding proteins. Twenty seven of these proteins affected reporter expression in the tethering screen. Our study provides novel insights into the potential trypanosome mRNPs composition, architecture and function.
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收藏
页码:457 / 471
页数:15
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