Cerebral Organoids-Challenges to Establish a Brain Prototype

被引：15
作者：
Eremeev, Artem, V [1 ,2 ]
Lebedeva, Olga S. [1 ,2 ]
Bogomiakova, Margarita E. [1 ,2 ]
Lagarkova, Maria A. [1 ,2 ]
Bogomazova, Alexandra N. [1 ,2 ]
机构：
[1] Fed Med Biol Agcy, Fed Res & Clin Ctr Phys Chem Med, Malaya Pirogovskaya 1a, Moscow 119435, Russia
[2] Fed Med Biol Agcy, Fed Res & Clin Ctr Phys Chem Med, Ctr Precis Genome Editing & Genet Technol Biomed, Malaya Pirogovskaya 1a, Moscow 119435, Russia
来源：
CELLS | 2021年 / 10卷 / 07期
基金：
俄罗斯科学基金会;
关键词：
iPSCs; brain organoids; differentiation; electrophysiology; neurotrophic factors; IN-VITRO; FUNCTIONAL MATURATION; HUMAN ES; DIRECTED DIFFERENTIATION; CORTICAL DEVELOPMENT; NEURAL PROGENITORS; CELL DIVERSITY; STEM-CELLS; RAT-BRAIN; CULTURE;
D O I：
10.3390/cells10071790
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The new cellular models based on neural cells differentiated from induced pluripotent stem cells have greatly enhanced our understanding of human nervous system development. Highly efficient protocols for the differentiation of iPSCs into different types of neural cells have allowed the creation of 2D models of many neurodegenerative diseases and nervous system development. However, the 2D culture of neurons is an imperfect model of the 3D brain tissue architecture represented by many functionally active cell types. The development of protocols for the differentiation of iPSCs into 3D cerebral organoids made it possible to establish a cellular model closest to native human brain tissue. Cerebral organoids are equally suitable for modeling various CNS pathologies, testing pharmacologically active substances, and utilization in regenerative medicine. Meanwhile, this technology is still at the initial stage of development.
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页数：17
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