Risk Assessment of Bovine Major Histocompatibility Complex Class II DRB3 Alleles for Perinatal Transmission of Bovine Leukemia Virus

被引：14

作者：

Borjigin, Liushiqi ^{[1
,2
]}

Lo, Chieh-Wen ^{[3
,4
]}

Bai, Lanlan ^{[1
,3
]}

Hamada, Rania ^{[3
,5
]}

Sato, Hirotaka ^{[1
,2
]}

Yoneyama, Shuji ^{[6
]}

Yasui, Anna ^{[7
]}

Yasuda, Sohei ^{[7
]}

Yamanaka, Risa ^{[7
]}

Mimura, Munehito ^{[7
]}

Inokuma, Michihito ^{[8
]}

Shinozaki, Yasuo ^{[9
]}

Tanaka, Naoko ^{[9
]}

Takeshima, Shin-Nosuke ^{[10
]}

Aida, Yoko ^{[1
,2
,4
]}

机构：

[1] RIKEN, Viral Infect Dis Unit, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[2] RIKEN Cluster Sci Technol & Innovat Hub, Baton Zone Program, Nakamura Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[3] RIKEN Ctr Adv Photon, Photon Control Technol Team, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[4] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Global Infect Dis Control Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

[5] South Valley Univ, Dept Anim Med, Fac Vet Med, Qena 83523, Egypt

[6] Kenou Livestock Hyg Serv Ctr, Utsunomiya, Tochigi 3210905, Japan

[7] Kumagaya Livestock Hyg Serv Ctr, Kumagaya, Saitama 3600813, Japan

[8] Chuo Livestock Hyg Serv Ctr, Chiba 2620011, Japan

[9] Nanbu Livestock Hyg Serv Ctr, Chiba 2960033, Japan

[10] Jumonji Univ, Dept Food & Nutr, Niiza, Saitama 3528510, Japan

来源：

PATHOGENS | 2021年 / 10卷 / 05期

关键词：

bovine leukemia virus; perinatal infection; dam; calf; BoLA-DRB3; polymorphism; proviral load; disease suitability; disease resistance; PROVIRAL LOAD; MILK-PRODUCTION; INFECTION; CATTLE; ASSOCIATION;

D O I：

10.3390/pathogens10050502

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Perinatal transmission plays a critical role in the spread of bovine leukemia virus (BLV) infection in cattle herds. In the Holstein breed, we previously identified BLV resistant and susceptible bovine leukocyte antigen (BoLA)-DRB3 alleles, including BoLA-DRB3*009:02 and *014:01:01 with a low BLV proviral load (PVL), and *015:01 and *012:01 with a high PVL. Here, we evaluated the perinatal BLV transmission risk in dams with different BoLA-DRB3 alleles. BoLA-DRB3 alleles of 120 dam-calf pairs from five dairy farms in Japan were identified; their PVL was quantified using the BLV-Coordination of Common Motifs (CoCoMo)-qPCR-2 assay. Ninety-six dams were BLV-positive, and 29 gave birth to BLV-infected calves. Perinatal transmission frequency was 19% in dams with resistant alleles suppressed to a low PVL level, and 38% and 25% in dams with susceptible and neutral alleles that maintained high PVL levels, respectively. Notably, all calves with resistant alleles were BLV free, whereas 30% of calves with susceptible genes were infected. Thus, vertical transmission risk was extremely lower for dams and calves with resistant alleles compared to those with susceptible alleles. Our results can inform the development of effective BLV eradication programs under field conditions by providing necessary data to allow for optimal selection of dams for breeding.

引用

页数：14

共 35 条

[1] Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus [J].