Establishment of human hepatocellular carcinoma CAM xenograft model and observation of angiogenesis characteristics

被引：0
作者：
Lv, Xiang [1 ]
Ling, Li [2 ]
Xia, Ying [1 ]
Xu, Yan-Feng [1 ]
Hou, Feng-Gang [1 ]
Ren, Jian-Lin [1 ]
Zhang, Xiao-Feng [1 ]
Li, Yan [1 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Shanghai Municipal Hosp Tradit Chinese Med, Shanghai 200071, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2016年 / 9卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Hepatoma; chick chorioallantoic membrane; models; animal; angiogenesis; EMBRYO CHORIOALLANTOIC MEMBRANE;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
To investigate the establishing method of human hepatoma cell line Bel-7402 CAM transplantation model and to observe and analyze the characteristics of angiogenesis. Different concentrations of human hepatoma Bel-7402 cells were inoculated on chick embryo chorioallantoic membrane (CAM) to observe CAM tumor survival factors, tumor growth characteristics and angiogenesis. Human hepatocellular carcinoma CAM xenograft model was established. In the model, tumor was easy to grow, with a strong angiogenic effect. The model is easy to copy, and can dynamically observe HCC angiogenesis process, which can be used to study the biological behavior of HCC and screen drugs.
引用
收藏
页码：3698 / 3703
页数：6
相关论文
共 50 条
  • [41] c7E3 Fab inhibits human tumor angiogenesis in a SCID mouse human skin xenograft model
    Nakada M.T.
    Cao G.
    Sassoli P.M.
    DeLisser H.M.
    Angiogenesis, 2006, 9 (4) : 171 - 176
  • [42] Inhibition of Tumor Growth of Human Hepatocellular Carcinoma HepG2 Cells in a Nude Mouse Xenograft Model by the Total Flavonoids from Arachniodes exilis
    Li, Huimin
    Jiang, Dengzhao
    Zhang, Lei
    Wu, Jiazhong
    EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2017, 2017
  • [43] Quantification in Molecular Ultrasound Imaging A Comparative Study in Mice Between Healthy Liver and a Human Hepatocellular Carcinoma Xenograft
    Sugimoto, Katsutoshi
    Moriyasu, Fuminori
    Negishi, Yoichi
    Hamano, Nobuhito
    Oshiro, Hisashi
    Rognin, Nicolas G.
    Yoshida, Tetsuya
    Kamiyama, Naohisa
    Aramaki, Yukihiko
    Imai, Yasuharu
    JOURNAL OF ULTRASOUND IN MEDICINE, 2012, 31 (12) : 1909 - 1916
  • [44] Comparing contrast-enhanced ultrasound to immunohistochemical markers of angiogenesis in a human melanoma xenograft model: Preliminary results
    Forsberg, F
    Dicker, AP
    Thakur, ML
    Rawool, NM
    Liu, JB
    Shi, WT
    Nazarian, LN
    ULTRASOUND IN MEDICINE AND BIOLOGY, 2002, 28 (04) : 445 - 451
  • [45] A new key player in VEGF-dependent angiogenesis in human hepatocellular carcinoma: dimethylarginine dimethylaminohydrolase 1
    Buijs, Nikki
    Oosterink, J. Efraim
    Jessup, Morgan
    Schierbeek, Henk
    Stolz, Donna B.
    Houdijk, Alexander P.
    Geller, David A.
    van Leeuwen, Paul A.
    ANGIOGENESIS, 2017, 20 (04) : 557 - 565
  • [46] A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice
    Zou, Yanmei
    Xiong, Hua
    Xiong, Huihua
    Lu, Tao
    Zhu, Feng
    Luo, Zhiyong
    Yuan, Xianglin
    Wang, Yihua
    TUMOR BIOLOGY, 2015, 36 (04) : 2929 - 2936
  • [47] Lenvatinib inhibits angiogenesis and tumor fibroblast growth factor signaling pathways in human hepatocellular carcinoma models
    Matsuki, Masahiro
    Hoshi, Taisuke
    Yamamoto, Yuji
    Ikemori-Kawada, Megumi
    Minoshima, Yukinori
    Funahashi, Yasuhiro
    Matsui, Junji
    CANCER MEDICINE, 2018, 7 (06): : 2641 - 2653
  • [48] Silencing cathepsin S gene expression inhibits growth, invasion and angiogenesis of human hepatocellular carcinoma in vitro
    Fan, Qi
    Wang, Xuedi
    Zhang, Hanguang
    Li, Chuanwei
    Fan, Junhua
    Xu, Jing
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 425 (04) : 703 - 710
  • [49] A new key player in VEGF-dependent angiogenesis in human hepatocellular carcinoma: dimethylarginine dimethylaminohydrolase 1
    Nikki Buijs
    J. Efraim Oosterink
    Morgan Jessup
    Henk Schierbeek
    Donna B. Stolz
    Alexander P. Houdijk
    David A. Geller
    Paul A. van Leeuwen
    Angiogenesis, 2017, 20 : 557 - 565
  • [50] Inhibition of human hepatocellular carcinoma tumor angiogenesis by siRNA silencing of VEGF via hepatic artery perfusion
    Zou, Y.
    Guo, C. -G.
    Zhang, M. -M.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2015, 19 (24) : 4751 - 4761
12345