Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

被引：24

作者：

Kumar, Ritesh ^{[1
]}

Singh, Amit Kumar ^{[1
]}

Lavania, Dhruv ^{[1
]}

Siddiqui, Manzer H. ^{[2
]}

Al-Whaibi, Mohamed H. ^{[2
]}

Grover, Anil ^{[1
]}

机构：

[1] Univ Delhi, Dept Plant Mol Biol, South Campus, New Delhi 110021, India

[2] King Saud Univ, Dept Bot & Microbiol, Riyadh, Saudi Arabia

来源：

SAUDI JOURNAL OF BIOLOGICAL SCIENCES | 2016年 / 23卷 / 02期

关键词：

ClpB; Faba bean; Heat stress; Hsp100; Pollen; Protein expression; Thermotolerance; Transcript induction; THERMOTOLERANCE; PLAYS;

D O I：

10.1016/j.sjbs.2015.03.006

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress. (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.

引用

页码：243 / 247

页数：5

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