UPLC-Q-TOF/MS based fecal metabolomics reveals the potential anti-diabetic effect of Xiexin Decoction on T2DM rats

被引：13

作者：

Zhang, Zhi-Miao ^{[1
]}

Chen, Meng-Jun ^{[1
]}

Zou, Jun-Feng ^{[1
]}

Jiang, Shu ^{[1
]}

Shang, Er-Xin ^{[1
]}

Qian, Da-Wei ^{[1
]}

Duan, Jin-Ao ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resour, 138 Xianlin Rd, Nanjing 210023, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2021年 / 1173卷

基金：

中国国家自然科学基金;

关键词：

Xiexin Decoction; Type 2 diabetes mellitus; UPLC-Q-TOF; MS; Metabolomics; TYPE-2; DIABETIC-RATS; BILE-ACID; METABOLISM; OBESITY; MODULATION; MICE;

D O I：

10.1016/j.jchromb.2021.122683

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Xiexin Decoction (XXD), a traditional Chinese medicine prescription composed of Rhei rhizome (RR), Scutellaria radix (SR) and Coptidis rhizome (CR), has been used to cure diabetes in clinical practices for thousands of years, but its mechanism is not clear. Our previous study indicated that XXD could significantly ameliorate the symptom of type 2 diabetes mellitus (T2DM) rats by shifting the composition of gut microbiota. However, the effect of XXD on the metabolic activity of gut microbiota is not clarified. In this study, the underlying mechanism of XXD on the amelioration of T2DM was explored by fecal metabolic profiling analysis based on ultra performance liquid chromatography coupled with quadrupole time-of-fight mass spectrometry (UPLC-Q-TOF/MS). The disordered metabolic profiles in T2DM rats were notably improved by XXD. Ten potential biomarkers, which were mainly involved in arachidonic acid metabolism, amino acid metabolism, bile acid metabolism, glycolysis and gluconeogenesis, were identified. Furthermore, these metabolites were closely related to SCFAs-producing and anti-inflammatory gut microflora. After XXD intervention, these biomarkers restored to the normal level at some extent. This study not only revealed potential biomarkers and related pathways in T2DM rats affected by XXD, but also provided a novel insight to uncover how traditional herb medicines worked from fecal metabolomics.

引用

页数：7