Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

被引：52

作者：

Fiuk, Agnieszka ^{[1
]}

Bednarek, Piotr T. ^{[2
]}

Rybczynski, Jan J. ^{[1
]}

机构：

[1] Polish Acad Sci, Bot Garden Ctr Biol Div Conservat, PL-02973 Warsaw, Poland

[2] Plant Breeding & Acclimatizat Inst, Dept Plant Biochem & Physiol, PL-05870 Blonie, Poland

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2010年 / 28卷 / 03期

关键词：

DNA methylation; Gentiana; Genetic stability; Somatic embryogenesis; ELAEIS-GUINEENSIS JACQ; SOMACLONAL VARIATION; DNA METHYLATION; CHROMOSOMAL INSTABILITY; ARABIDOPSIS-THALIANA; CALLUS-CULTURE; CELL-CULTURES; AFLP ANALYSIS; VULGARE L; POLYMORPHISM;

D O I：

10.1007/s11105-009-0167-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytometric and molecular techniques were used to verify genetic uniformity among somatic embryo-derived plantlets of Gentiana pannonica Scop. Cytometric analysis of regenerants revealed absence of chromosomal changes and alterations in ploidy. However, reverse phase high pressure liquid chromatography detected higher levels of methylation in regenerated plants than those of control plants. These changes were further investigated using a quantitative molecular marker-based approach. This revealed that numerous tissue culture-induced variations, similar to 3% (epi)mutations, were observed, including sequence variation and changes in methylation patterns. Moreover, complex patterns of variation, including combinations of genetic and epigenetic changes, were relatively high (ca. 9%). Overall, tissue culture-induced variation reached 16%; while, demethylation was lower than de novo methylation in heterozygotic material and similar in all regenerated plantlets.

引用

页码：413 / 420

页数：8

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