Photo-Oxidative Stress on Model Membranes - Studies by Optical Methods

被引:0
作者
Barbinta Patrascu, M. E. [1 ]
Badea, Nicoleta Maria [2 ]
Tugulea, Laura [1 ]
Meghea, Aurelia [2 ]
机构
[1] Univ Bucharest, Fac Phys, Magurele 077125, Romania
[2] Univ POLITEH Bucharest, Fac Appl Chem & Mat Sci, Bucharest, Romania
来源
ELECTROCHEMISTRY AND PHYSICAL CHEMICAL METHODS IN SERVING MATERIALS FOR SUSTAINABLE DEVELOPMENT | 2009年 / 415卷
关键词
photo-oxidative stress; chlorophyll a; beta-carotene; alpha-tocopherol-acetate; quercetin; liposomes; CAROTENOIDS;
D O I
暂无
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Photophysical studies on oxidative stress induced by illumination with ultraviolet (UV) light from artificial light sources, were performed on small unilamellar liposomes (SUVs) as models of biomembranes. Different liposoluble antioxidants were incorporated in the liposome bilayer during the lipid film preparation. The quercetin containing bilayers exhibited a high tolerance to photooxidative stress. The light-driven reactive oxygen species (ROS) effects on liposomal membranes were monitored by optical methods: UV-VIS absorption, fluorescence and chemiluminescence (CL), exploiting the spectral properties of chlorophyll a (Chla) embedded into some liposome bilayers (Chla/lipid molar ratio = 1/100). The interaction between Chla and the other liposome components resulted in changes in their absorption and emission fluorescence properties.
引用
收藏
页码:29 / +
页数:2
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