Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l.

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its specks are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of specks diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E conehaturn, E flabellaium, gregarium, F. pleurotoides and E reductum. Molecular phylogeny of Entoloma 51 was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus,"Alboleptonia", Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clacks. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoid.es-group, previously considered within Claudopus, formed a separate lade; but section Claudopus and relatives of F. undaturn belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Eniolorna s.l. paraphyletic.