Somatic embryogenesis of Tinospora cordifolia using in vitro leaf explants

This study investigates factors affecting the production of somatic embryos in Tinospora cordifolia. Fully expanded leaves taken from in-vitro grown plants were used as explants. The explants were cultured on Murashige and Skoog medium supplemented with different concentrations of NAA or 2, 4-D. Explants grown on medium supplemented with 21.48uM NAA gave callus with organized globular protuberances. Somatic embryos when cultured on media containing ABA matured and gave rise to a number of secondary somatic embryos. These somatic embryos germinated when transferred to half strength MS medium supplemented with 5.77uM GA(3) and 2.32uM Kinetin.