DEVELOPMENT, CHARACTERIZATION, AND TRANSFERABILITY OF MICROSATELLITE MARKERS FOR KIRENGESHOMA PALMATA (HYDRANGEACEAE)

被引：6

作者：

Sun, Yi ^{[1
,2
,3
]}

Fu, Cheng-Xing ^{[1
,2
,3
]}

Qiu, Ying-Xiong ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Minist Educ, Conservat Ctr Gene Resources Endangered Wildlife, Hangzhou 310058, Zhejiang, Peoples R China

[3] Minist Educ, Key Lab Conservat Genet & Reprod Biol Endangered, Hangzhou 310058, Zhejiang, Peoples R China

来源：

AMERICAN JOURNAL OF BOTANY | 2010年 / 97卷 / 06期

基金：

美国国家科学基金会;

关键词：

endangered species; Kirengeshoma; mating system; microsatellite; GENETIC DIVERSITY; SAXIFRAGACEAE;

D O I：

10.3732/ajb.1000134

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Microsatellite markers were developed for Kirengeshoma palmata to assess the population genetics and mating pattern of this critically endangered species. Methods and Results: A total of 24 microsatellite markers were developed for K. palmata using an enrichment protocol. These markers were screened in 37 individuals from four populations in China and Japan, and twelve were found to be polymorphic, with the number of alleles per locus ranging from two to eight. All of these primers also amplified in K. koreana. Conclusions: These microsatellite markers provide a useful tool to investigate the mating system, gene flow, parentage, and population dynamics of Kirengeshoma.

引用

页码：E48 / E51

页数：4

共 9 条

[1] Genetic diversity and mating system of the threatened plant Kirengeshoma palmata (Saxifragaceae) in Korea [J].

Chang, Chin-Sung ;

Choi, Do Yol ;

Kim, Hui ;

Kim, Yong-Shik ;

Park, Tae Yoon .

JOURNAL OF PLANT RESEARCH, 2007, 120 (01) :149-156

[2]

Clarke K.R., 2001, PRIMER V5 USER MANUA

[3]

Doyle J. J., 1991, Molecular techniques in taxonomy., P283

[4] Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment [J].

Kalinowski, Steven T. ;

Taper, Mark L. ;

Marshall, Tristan C. .

MOLECULAR ECOLOGY, 2007, 16 (05) :1099-1106

[5] Large-scale processes and the Asian bias in species diversity of temperate plants [J].

Qian, H ;

Ricklefs, RE .

NATURE, 2000, 407 (6801) :180-182

[6] Molecular phylogeography of East Asian Kirengeshoma (Hydrangeaceae) in relation to Quaternary climate change and landbridge configurations [J].

Qiu, Ying-Xiong ;

Sun, Yi ;

Zhang, Xiao-Ping ;

Lee, Joongku ;

Fu, Cheng-Xin ;

Comes, Hans Peter .

NEW PHYTOLOGIST, 2009, 183 (02) :480-495

[7] GENEPOP′007:: a complete re-implementation of the GENEPOP software for Windows and Linux [J].

Rousset, Francois .

MOLECULAR ECOLOGY RESOURCES, 2008, 8 (01) :103-106

[8] Strategies for microsatellite isolation: a review [J].

Zane, L ;

Bargelloni, L ;

Patarnello, T .

MOLECULAR ECOLOGY, 2002, 11 (01) :1-16

[9] Genetic diversity of the endangered species Kirengeshoma palmata (Saxifragaceae) in China [J].

Zhang, XP ;

Li, XH ;

Qiu, YX .

BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2006, 34 (01) :38-47

← 1 →