Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

被引：116

作者：

Gao, Manchen ^{[1
]}

Zhang, Hengyi ^{[1
]}

Dong, Wei ^{[1
]}

Bai, Jie ^{[1
,2
]}

Gao, Botao ^{[1
]}

Xia, Dekai ^{[1
]}

Feng, Bei ^{[1
,2
]}

Chen, Maolin ^{[1
]}

He, Xiaomin ^{[1
]}

Yin, Meng ^{[1
]}

Xu, Zhiwei ^{[1
]}

Witman, Nevin ^{[3
]}

Fu, Wei ^{[1
,2
]}

Zheng, Jinghao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Dept Cardiothorac Surg, Sch Med, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Inst Pediat Translat Med, Sch Med, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China

[3] Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

AIRWAY RECONSTRUCTION; GRAFT; MANAGEMENT; STENOSIS; CELLS;

D O I：

10.1038/s41598-017-05518-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D printed a biodegradable reticular polycaprolactone (PCL) scaffold with similar morphology to the whole segment of rabbits' native trachea. The 3D-printed scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits' native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 +/- 5.42 days, and in Group II was 22.58 +/- 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D printed TET.

引用

页数：12

共 30 条

[1]

Abdulcemal Isik U, 2002, J Cardiovasc Surg (Torino), V43, P281

[2]

Agarwala S., 2016, 2 INT C PROGR ADD MA

[3]

Agarwala S., 2016, AM J ENG APPL SCI, V9, P985, DOI [10.3844/ajeassp.2016, DOI 10.3844/AJEASSP.2016.985.990]

[4] Management of congenital tracheal stenosis in infancy [J].