Functional fibrous polypropylene solid support and its application in solid phase peptide synthesis and cell specific binding

被引：4
|
作者：
Xiang, Bei [2 ]
Lam, Kit S. [1 ]
Sun, Gang [3 ]
机构：
[1] Univ Calif Davis, Dept Internal Med, Div Hematol & Oncol, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
[3] Univ Calif Davis, Div Text & Clothing, Davis, CA 95616 USA
来源：
REACTIVE & FUNCTIONAL POLYMERS | 2009年 / 69卷 / 12期
基金：
美国国家科学基金会;
关键词：
Polypropylene fibers; Radical grafting; Solid support; Peptide synthesis; COMBINATORIAL LIBRARY METHOD; SURFACE MODIFICATION; MALEIC-ANHYDRIDE; PROTEIN MICROARRAYS; CELLULOSE PAPER; SPOT-SYNTHESIS; ACRYLIC-ACID; POLYETHYLENE; IMMOBILIZATION; POLYMERIZATION;
D O I：
10.1016/j.reactfunctpolym.2009.09.007
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Surfaces of polypropylene (PP) fabrics were modified through thermal initiated radical graft polymerization with acrylic acid and poly (ethylene glycol) di-acrylate (PEG-DIA). Various polymerization conditions were studied to determine the optimal parameters leading to the best surface functionality. Carboxylic acid groups on the functionalized PP support were further derivatized for solid phase peptide synthesis. Peptide ligands LHPQF and sppLDI were successfully synthesized through simple coupling reactions utilizing Fmoc chemistry. Specific binding of Streptavidin-Alkaline phosphatase conjugate and Jurkat (T lymphoblastic) cell were successfully achieved on the novel functional PP substrates. The good geometrical flexibility, mechanical stability, chemical reactivity and biocompatibility of this novel planar support make it very promising substrate for various biotechnological applications. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：905 / 914
页数：10
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