Isocratic high-performance liquid chromatographic method for the separation of isradipine and its main metabolites Application to in vitro metabolization by h3A4/OR cells

被引：4

作者：

Bidouil, S

Dubois, J

Hanocq, M

机构：

[1] Université Libre de Bruxelles, Institut de Pharmacie, Lab. Chim. Bioanalytique Toxicologie, 1050 Brussels, Boulevard du Triomphe

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 02期

关键词：

h3A4/OR cells; isradipine;

D O I：

10.1016/S0378-4347(97)00048-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase HPLC method was developed for the study of isradipine oxidation in vitro. The drug and its main metabolites were determined after extraction from culture media and from h3A4/OR cells having the cytochrome P450 isoenzyme involved in the xenobiotic metabolization. The HPLC assay was fully validated and was used to follow the biotransformation kinetics of isradipine.

引用

页码：359 / 366

页数：8

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