A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters

被引：35

作者：

Koseki, Takuya ^{[1
]}

Mihara, Koji ^{[1
]}

Murayama, Tetsuya ^{[1
]}

Shiono, Yoshihito ^{[1
]}

机构：

[1] Yamagata Univ, Dept Bioresource Engn, Fac Agr, Tsuruoka, Yamagata 9978555, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 18期

基金：

日本学术振兴会;

关键词：

Esterase; 4-Hydroxybenzoic acid ester; Paraben; Aspergillus oryzae; FERULOYL ESTERASE; ENTEROBACTER-CLOACAE; PURIFICATION; PARABENS; NIGER; DEGRADATION; PROTEINS; AWAMORI; GENES; PRBA;

D O I：

10.1016/j.febslet.2010.08.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we report the biochemical characterization of a hypothetical protein from Aspergillus oryzae exhibiting sequence identity with feruloyl esterase and tannase from the genus Aspergillus. The purified recombinant protein showed a hydrolytic activity toward the ethyl, propyl, or butyl esters of 4-hydroxybenzoic acid, but did not show feruloyl esterase or tannase activity. Finally, the enzyme decreased the antimicrobial activity of parabens against A. oryzae via hydrolysis of the ester bond present in butyl 4-hydroxybenzoic acid. (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4032 / 4036

页数：5

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