Kinin B1 Receptor Enhances the Oxidative Stress in a Rat Model of Insulin Resistance: Outcome in Hypertension, Allodynia and Metabolic Complications

被引:53
作者
Dias, Jenny Pena [1 ]
Talbot, Sebastien [1 ]
Senecal, Jacques [1 ]
Carayon, Pierre [2 ]
Couture, Rejean [1 ]
机构
[1] Univ Montreal, Fac Med, Dept Physiol, Montreal, PQ H3C 3J7, Canada
[2] Sanofi Aventis R&D, Montpellier, France
来源
PLOS ONE | 2010年 / 5卷 / 09期
基金
加拿大健康研究院;
关键词
CONVERTING ENZYME-INHIBITION; ALPHA-LIPOIC ACID; ANGIOTENSIN-II; SUPEROXIDE-PRODUCTION; BRADYKININ B1; PHARMACOLOGICAL BLOCKADE; SENSORY ABNORMALITIES; MYOCARDIAL-ISCHEMIA; ANTIOXIDANT ENZYMES; OXIDASE ACTIVATION;
D O I
10.1371/journal.pone.0012622
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Kinin B-1 receptor (B1R) is induced by the oxidative stress in models of diabetes mellitus. This study aims at determining whether B1R activation could perpetuate the oxidative stress which leads to diabetic complications. Methods and Findings: Young Sprague-Dawley rats were fed with 10% D-Glucose or tap water (controls) for 8-12 weeks. A selective B1R antagonist (SSR240612) was administered acutely (3-30 mg/kg) or daily for a period of 7 days (10 mg/kg) and the impact was measured on systolic blood pressure, allodynia, protein and/or mRNA B1R expression, aortic superoxide anion (O-2(center dot-)) production and expression of superoxide dismutase (MnSOD) and catalase. SSR240612 reduced dose-dependently (3-30 mg/kg) high blood pressure in 12-week glucose-fed rats, but had no effect in controls. Eight-week glucose-fed rats exhibited insulin resistance (HOMA index), hypertension, tactile and cold allodynia and significant increases of plasma levels of glucose and insulin. This was associated with higher aortic levels of O-2(center dot-), NADPH oxidase activity, MnSOD and catalase expression. All these abnormalities including B1R overexpression (spinal cord, aorta, liver and gastrocnemius muscle) were normalized by the prolonged treatment with SSR240612. The production of O-2(center dot-) in the aorta of glucose-fed rats was also measured in the presence and absence of inhibitors (10-100 mu M) of NADPH oxidase (apocynin), xanthine oxidase (allopurinol) or nitric oxide synthase (L-NAME) with and without Sar[D-Phe(8)]des-Arg(9)-BK (20 mu M; B1R agonist). Data show that the greater aortic O-2(center dot-) production induced by the B1R agonist was blocked only by apocynin. Conclusions: Activation of kinin B1R increased O-2(center dot-) through the activation of NADPH oxidase in the vasculature. Prolonged blockade of B1R restored cardiovascular, sensory and metabolic abnormalities by reducing oxidative stress and B1R gene expression in this model.
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页码:1 / 14
页数:14
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