Electrochemical immunosensor assay (EIA) for sensitive detection of E. coli O157:H7 with signal amplification on a SG-PEDOT-AuNPs electrode interface

被引：43

作者：

Guo, Yuna ^{[1
]}

Wang, Yu ^{[2
]}

Liu, Su ^{[3
]}

Yu, Jinghua ^{[1
]}

Wang, Hongzhi ^{[1
]}

Cui, Min ^{[1
]}

Huang, Jiadong ^{[1
,2
]}

机构：

[1] Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China

[2] Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Peoples R China

[3] Univ Jinan, Sch Resources & Environm, Jinan 250022, Peoples R China

来源：

ANALYST | 2015年 / 140卷 / 02期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

ESCHERICHIA-COLI; GOLD NANOPARTICLES; BIOSENSOR; RECOGNITION; ENUMERATION;

D O I：

10.1039/c4an01463d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemical immunosensor assay (EIA) for highly sensitive and specific detection of Escherichia coli O157:H7 has been developed. This immunosensor is constructed by the assembly of capture antibody on SG-PEDOT-AuNPs composites modified glass carbon electrode. In the presence of target E. coli O157:H7, horseradish peroxidase (HRP)-labeled antibody is captured on the electrode surface to form a sandwich-type system via the specific identification. As a result, E. coli O157:H7 detection is realized by outputting a redox current from electro-reduction of hydrogen peroxide reaction catalyzed by HRP. In our assay, the combination of the unique properties of sulfonated graphene (SG) and gold nanoparticles (AuNPs) can not only accelerate electron transfer on electrode interface, but also provide an excellent scaffold for the conjugation of capture antibody that significantly improves the target capture efficiency and enhances the sensitivity of the biosensor. The results reveal the calibration plot obtained for E. coli O157:H7 is approximately linear from 7.8 x 10-7.8 x 10(6) colony-forming unit (cfu) mL(-1) with the limit of detection of 3.4 x 10 cfu mL(-1). In addition, the biosensor has been successfully applied to the quantitative assay of E. coli O157:H7 in synthetic samples (spring water and milk). Hence, the developed electrochemical-based immunosensor might provide a useful and practical tool for E. coli O157:H7 determination and related food safety analysis and clinical diagnosis.

引用

页码：551 / 559

页数：9

共 41 条

[21] 4-Amino-1-(3-mercapto-propyl)-pyridine Hexafluorophosphate Ionic Liquid Functionalized Gold Nanoparticles for IgG Immunosensing Enhancement [J].

Li, Rui ;

Wu, Kangbing ;

Liu, Changxian ;

Huang, Yin ;

Wang, Yanying ;

Fang, Huaifang ;

Zhang, Huijuan ;

Li, Chunya .

ANALYTICAL CHEMISTRY, 2014, 86 (11) :5300-5307

[22] A sensitive and selective nitrite sensor based on a glassy carbon electrode modified with gold nanoparticles and sulfonated graphene [J].

Li, Su-Juan ;

Zhao, Guo-Yan ;

Zhang, Rong-Xia ;

Hou, Ya-Li ;

Liu, Lin ;

Pang, Huan .

MICROCHIMICA ACTA, 2013, 180 (9-10) :821-827

[23] Supersandwich Cytosensor for Selective and Ultrasensitive Detection of Cancer Cells Using Aptamer-DNA Concatamer-Quantum Dots Probes [J].

Liu, Hongying ;

Xu, Shouming ;

He, Zhimei ;

Deng, Anping ;

Zhu, Jun-Jie .

ANALYTICAL CHEMISTRY, 2013, 85 (06) :3385-3392

[24] Synthesis of Potent Inhibitors of β-Ketoacyl-Acyl Carrier Protein Synthase III as Potential Antimicrobial Agents [J].

Liu, Yan ;

Zhong, Wu ;

Li, Rui-Juan ;

Li, Song .

MOLECULES, 2012, 17 (05) :4770-4781

[25] Wireless, Remote-Query, and High Sensitivity Escherichia coli O157:H7 Biosensor Based on the Recognition Action of Concanavalin A [J].

Lu, Qingzhu ;

Lin, Hailan ;

Ge, Shutian ;

Luo, Shenglian ;

Cai, Qingyun ;

Grimes, Craig A. .

ANALYTICAL CHEMISTRY, 2009, 81 (14) :5846-5850

[26] Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces [J].

Luedtke, Brandon E. ;

Bono, James L. ;

Bosilevac, Joseph M. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2014, 105 :72-79

[27] A method for DNA-based detection of E. coli O157:H7 in a proteinous background using piezoelectric-excited cantilever sensors [J].

Rijal, Kishan ;

Mutharasan, Raj .

ANALYST, 2013, 138 (10) :2943-2950

[28] hlyA Gene-Based Sensitive Detection of Listeria monocytogenes Using a Novel Cantilever Sensor [J].

Sharma, Harsh ;

Mutharasan, Raj .

ANALYTICAL CHEMISTRY, 2013, 85 (06) :3222-3228

[29] Enzymatic biosensors based on the use of metal oxide nanoparticles [J].

Shi, Xinhao ;

Gu, Wei ;

Li, Bingyu ;

Chen, Ningning ;

Zhao, Kai ;

Xian, Yuezhong .

MICROCHIMICA ACTA, 2014, 181 (1-2) :1-22

[30] Synthesis of water soluble graphene [J].

Si, Yongchao ;

Samulski, Edward T. .

NANO LETTERS, 2008, 8 (06) :1679-1682

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