Toxoplasma gondii:: DNA vaccination with genes encoding antigens MIC2, WAP, AMA1 and BAG1 and evaluation of their immunogenic potential

被引：56
作者：
Dautu, George
Munyaka, Biscah
Carmen, Gabriella
Zhang, Guohong
Omata, Yoshitaka
Xuenan, Xuan
Igarashi, Makoto [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Kenya Govt Med Res Ctr, Nairobi 5484000200, Kenya
[3] Serv Nacl Salud & Calidad Anim, San lorenzo, Paraguay
[4] Obihiro Univ Agr & Vet Med, Sch Vet Med, Dept Vet Basic Sci, Obihiro, Hokkaido 0808555, Japan
来源：
EXPERIMENTAL PARASITOLOGY | 2007年 / 116卷 / 03期
基金：
日本学术振兴会;
关键词：
Toxoplasma gondii; DNA immunization; PROTECTIVE IMMUNITY; INTERFERON-GAMMA; ANTIBODY-RESPONSES; MICRONEME PROTEINS; PREGNANT-WOMEN; T-LYMPHOCYTE; IMMUNIZATION; INFECTION; PARASITE; INDUCTION;
D O I：
10.1016/j.exppara.2007.01.017
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-gamma synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes. (c) 2007 Elsevier Inc. All rights reserved.
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收藏
页码：273 / 282
页数：10
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