Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus

被引：0
|
作者：
Li, Shikai [1 ,2 ]
Wang, Wei [1 ,2 ]
Yang, Mingju [1 ,2 ]
Huang, Fujiang [1 ,2 ]
Wang, Xue [1 ,2 ]
Zeng, Sheng [1 ,2 ]
Xiang, Yan [1 ,2 ]
Zhou, Lu [1 ,2 ]
机构：
[1] Guizhou Acad Agr Sci, Fisheries Res Inst, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
[2] Engn & Technol Ctr Special Fisheries, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
来源：
CONSERVATION GENETICS RESOURCES | 2022年 / 14卷 / 03期
关键词：
Semilabeo obscurus; SNP; Molecular marker; Germplasm conservation;
D O I：
10.1007/s12686-022-01271-6
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Semilabeo obscurus, as an endangered species, is a certain economic fish in China and Vietnam. However, study on its genetic diversity is still lacking. In this study, 168 candidate SNPs were verified by Sanger-sequencing. Of these, 123 polymorphic SNP markers were successfully genotyped. The observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information conten (PIC) ranged from 0.0968-0.9310 (mean 0.3590), 0.0936-0.5085 (mean 0.3550) and 0.0879-0.3750 (mean 0.2807), respectively. Only 3 SNPs (Sob-016, Sob-050 and Sob-115) were significantly deviated from Hardy-Weinberg equilibrium (P < 0.05). Our study may help researchers better understand the genetic traits of S. obscurus,and provide reference for further protecting and reasonably utilizing its genetic resources.
引用
收藏
页码：257 / 261
页数：5
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