Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1 beta, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods: Twenty male Wistar rats divided to four groups, included, control group with normal diet (C+ND), OVA-sensitized group with normal diet (S+ND), control group with high-fat diet (C+HFD), and OVA-sensitized group with high-fat diet (S+HFD). All animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks with designed regimens. At the end of study, trachea isolated and examined for expression levels of IL-1 beta, IRAK-1 and TRAF-6 mRNA with RT-PCR method. Results: Diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (P< 0.05 to P< 0.001). The expression levels of IL-1 beta mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significantly increased when compared with control group. Also in S+HFD group, expression level of TRAF-6 mRNA was higher than other groups (P< 0.001). IRAK-1 expression level was high in S+HFD compared with control group. IL-1 beta and TRAF-6 mRNA correlated positively with obesity indexes. Conclusion: The results showed that DIO causes overexpression of IL-1 beta, IRAK-1 and TRAF-6 mRNA in an experimental model of asthma. Our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. These findings showed that possible mechanism for obesity-asthma relationships.
机构:
Tabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Tabriz Univ Med Sci, Dept Physiol, Fac Med, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Alipour, Mohammad Reza
Khamaneh, Amir Mahdi
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Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol RCPN, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Khamaneh, Amir Mahdi
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Yousefzadeh, Nasibeh
Mohammad-nejad, Daryoush
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Tabriz Univ Med Sci, Dept Histol, Fac Med, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Mohammad-nejad, Daryoush
Soufi, Farhad Ghadiri
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Hormozgan Univ Med Sci, Res Ctr Mol Med, Bandar Abbas, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
机构:
Tabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Tabriz Univ Med Sci, Dept Physiol, Fac Med, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Alipour, Mohammad Reza
Khamaneh, Amir Mahdi
论文数: 0引用数: 0
h-index: 0
机构:
Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol RCPN, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Khamaneh, Amir Mahdi
论文数: 引用数:
h-index:
机构:
Yousefzadeh, Nasibeh
Mohammad-nejad, Daryoush
论文数: 0引用数: 0
h-index: 0
机构:
Tabriz Univ Med Sci, Dept Histol, Fac Med, Tabriz, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran
Mohammad-nejad, Daryoush
Soufi, Farhad Ghadiri
论文数: 0引用数: 0
h-index: 0
机构:
Hormozgan Univ Med Sci, Res Ctr Mol Med, Bandar Abbas, IranTabriz Univ Med Sci, Neurosci Res Ctr, Tabriz, Iran