Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats

被引：43

作者：

Althunibat, Osama Y. ^{[1
]}

Al-Mustafa, Ahmed H. ^{[2
]}

Tarawneh, Khaled ^{[2
]}

Khleifat, Khaled M. ^{[2
]}

Ridzwan, B. H. ^{[1
]}

Qaralleh, Haitham N. ^{[1
]}

机构：

[1] Int Islamic Univ Malaysia, Dept Biomed Sci, Kuantan 25200, Pahang, Malaysia

[2] Mutah Univ, Dept Biol, Mutah, Karak, Jordan

来源：

PROCESS BIOCHEMISTRY | 2010年 / 45卷 / 04期

关键词：

Punica granatum; Diabetes; Streptozotocin; Antioxidant; Malondialdehyde; Jordan; ANTIOXIDANT ACTIVITY; BLOOD-GLUCOSE; VANADATE; MANAGEMENT; GLYCATION;

D O I：

10.1016/j.procbio.2009.12.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Punica granatum L. (Punicaceae) peels extract had the highest free radical scavenging capacity among the tested medicinal plants which are being used traditionally for treatment of diabetes in Jordan. Accordingly, the present study aimed to investigate the antioxidant effect of P. granatum peel methanolic extract against oxidative damage in streptozotocin-induced diabetic rats. The antioxidant activity of P. granatum peel extract was investigated by examining the level of antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR), the serum total antioxidant capacity and lipid peroxidation in the tissues of treated diabetic rates comparing with normal and untreated diabetic ones. The results revealed that intraperitoneal administration of 10 and 20 mg kg(-1) (body weight) of P. granatum peel extract for 4 weeks significantly enhanced the activities of antioxidant enzymes in liver, kidney and RBC of STZ-induced diabetic rats. The extract also caused a significant reduction in malondialdehyde (MDA), a lipid peroxide's marker, in diabetic rat tissues and elevated the total serum antioxidant capacity in dose-dependent manner. In conclusion, this study clearly showed that P. granatum peel extract has protective role against the oxidative damage in STZ-induced diabetic rats. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：581 / 585

页数：5

共 36 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2] Anthocyanin- and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-κB pathways and inhibits skin tumorigenesis in CD-1 mice [J].

Afaq, F ;

Saleem, M ;

Krueger, CG ;

Reed, JD ;

Mukhtar, H .

INTERNATIONAL JOURNAL OF CANCER, 2005, 113 (03) :423-433

[3]

Al-Mustafa Ahmed H, 2008, Pak J Biol Sci, V11, P351, DOI 10.3923/pjbs.2008.351.358

[4] Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats [J].

Andallu, B ;

Varadacharyulu, NC .

CLINICA CHIMICA ACTA, 2003, 338 (1-2) :3-10

[5] ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES [J].

BAYNES, JW .

DIABETES, 1991, 40 (04) :405-412

[6] INVIVO INSULIN RESISTANCE IN STREPTOZOTOCIN-DIABETIC RATS - EVIDENCE FOR REVERSAL FOLLOWING ORAL VANADATE TREATMENT [J].

BLONDEL, O ;

BAILBE, D ;

PORTHA, B .

DIABETOLOGIA, 1989, 32 (03) :185-190

[7]

Chattopadhyay S., 1997, Indian Journal of Experimental Biology, V35, P1141

[8] Studies on antidiarrhoeal activity of Punica granatum seed extract in rats [J].

Das, AK ;

Mandal, SC ;

Banerjee, SK ;

Sinha, S ;

Das, J ;

Saha, BP ;

Pal, M .

JOURNAL OF ETHNOPHARMACOLOGY, 1999, 68 (1-3) :205-208

[9]

DRAPER HH, 1990, METHOD ENZYMOL, V186, P421

[10]

DUBYAK GR, 1980, J BIOL CHEM, V255, P5306

← 1 2 3 4 →