Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins

被引：34
作者：
Knöpfel, T [1 ]
Tomita, K [1 ]
Shimazaki, R [1 ]
Sakai, R [1 ]
机构：
[1] RIKEN, Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama 3510198, Japan
来源：
METHODS | 2003年 / 30卷 / 01期
关键词：
green fluorescent protein; optical imaging; voltage-sensitive dyes; biosensors; fluorescence resonance energy transfer;
D O I：
10.1016/S1046-2023(03)00006-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：42 / 48
页数：7
相关论文
共 29 条
  • [11] Reducing the environmental sensitivity of yellow fluorescent protein - Mechanism and applications
    Griesbeck, O
    Baird, GS
    Campbell, RE
    Zacharias, DA
    Tsien, RY
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) : 29188 - 29194
  • [12] VISUALIZATION OF THE SPREAD OF ELECTRICAL-ACTIVITY IN RAT HIPPOCAMPAL SLICES BY VOLTAGE-SENSITIVE OPTICAL PROBES
    GRINVALD, A
    MANKER, A
    SEGAL, M
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1982, 333 (DEC): : 269 - 291
  • [13] Green fluorescent protein as a noninvasive intracellular pH indicator
    Kneen, M
    Farinas, J
    Li, YX
    Verkman, AS
    [J]. BIOPHYSICAL JOURNAL, 1998, 74 (03) : 1591 - 1599
  • [14] Crystal structure of the tetramerization domain of the Shaker potassium channel
    Kreusch, A
    Pfaffinger, PJ
    Stevens, CF
    Choe, S
    [J]. NATURE, 1998, 392 (6679) : 945 - 948
  • [15] T1-T1 interactions occur in ER membranes while nascent Kv peptides are still attached to ribosomes
    Lu, JL
    Robinson, JM
    Edwards, D
    Deutsch, C
    [J]. BIOCHEMISTRY, 2001, 40 (37) : 10934 - 10946
  • [16] Fluorescent proteins from nonbioluminescent Anthozoa species
    Matz, MV
    Fradkov, AF
    Labas, YA
    Savitsky, AP
    Zaraisky, AG
    Markelov, ML
    Lukyanov, SA
    [J]. NATURE BIOTECHNOLOGY, 1999, 17 (10) : 969 - 973
  • [17] Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter
    Metzger, F
    Repunte-Canonigo, V
    Matsushita, S
    Akemann, W
    Diez-Garcia, J
    Ho, CS
    Iwasato, T
    Grandes, P
    Itohara, S
    Joho, RH
    Knöpfel, T
    [J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2002, 15 (01) : 40 - 50
  • [18] Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein
    Pearce, LL
    Gandley, RE
    Han, WP
    Wasserloos, K
    Stitt, M
    Kanai, AJ
    McLaughlin, MK
    Pitt, BR
    Levitan, ES
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (01) : 477 - 482
  • [19] Using GFP in FRET-based applications
    Pollok, BA
    Heim, R
    [J]. TRENDS IN CELL BIOLOGY, 1999, 9 (02) : 57 - 60
  • [20] Structure and emergence of specific olfactory glomeruli in the mouse
    Potter, SM
    Zheng, C
    Koos, DS
    Feinstein, P
    Fraser, SE
    Mombaerts, P
    [J]. JOURNAL OF NEUROSCIENCE, 2001, 21 (24) : 9713 - 9723
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