RETRACTED: A photoactivatable green-fluorescent protein from the phylum Ctenophora (Retracted Article)

被引:22
作者
Haddock, Steven H. D. [1 ]
Mastroianni, Nadia [2 ,3 ]
Christianson, Lynne M. [1 ]
机构
[1] Monterey Bay Aquarium Res Inst, Moss Landing, CA 95039 USA
[2] Axxam SpA, I-20132 Milan, Italy
[3] Farm Posca, I-22060 Carugo, Co, Italy
关键词
comb jelly; GFP; bioluminescence; fluorescence; photoactivation; EVOLUTION; GFP; DIVERSITY; AMPLIFICATION; ANTHOZOA; YIELD; MODEL;
D O I
10.1098/rspb.2009.1774
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Genes for the family of green-fluorescent proteins (GFPs) have been found in more than 100 species of animals, with some species containing six or more copies producing a variety of colours. Thus far, however, these species have all been within three phyla: Cnidaria, Arthropoda and Chordata. We have discovered GFP-type fluorescent proteins in the phylum Ctenophora, the comb jellies. The ctenophore proteins share the xYG chromophore motif of all other characterized GFP-type proteins. These proteins exhibit the uncommon property of reversible photoactivation, in which fluorescent emission becomes brighter upon exposure to light, then gradually decays to a non-fluorescent state. In addition to providing potentially useful optical probes with novel properties, finding a fluorescent protein in one of the earliest diverging metazoans adds further support to the possibility that these genes are likely to occur throughout animals.
引用
收藏
页码:1155 / 1160
页数:6
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