Application of immunological methods for the detection of species adulteration in dairy products

被引：64

作者：

Hurley, IP ^{[1
]}

Ireland, HE ^{[1
]}

Coleman, RC ^{[1
]}

Williams, JHH ^{[1
]}

机构：

[1] Univ Coll Chester, Dept Biol Sci, Chester Ctr Stress Res, Chester CH1 4BJ, Cheshire, England

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2004年 / 39卷 / 08期

关键词：

enzyme-linked immunosorbent assay; immunoassay; milk adulteration;

D O I：

10.1111/j.1365-2621.2004.00861.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A number of enzyme-linked immunosorbent assays (ELISAs) have been developed for the detection of milk adulteration in dairy products. Target antigens have been caseins, lactoglobulins, immunoglobulins and other whey proteins. Polyclonal and monoclonal antibodies have been used in a variety of formats including direct, indirect, competitive and sandwich ELISAs. ELISAs have been successfully applied to the detection of cows' milk adulteration of sheep, goat and buffalo milk. Goat milk adulteration of sheep milk has also been detected. A number of ELISAs have also been applied to cheese. It is recommended that ELISA should be used in combination with PCR to ensure compliance with current legislation.

引用

页码：873 / 878

页数：6

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