Isolation and characterization of microsatellite loci in the black rat snake (Elaphe obsoleta)

被引：12
|
作者：
Blouin-Demers, G [1 ]
Gibbs, HL [1 ]
机构：
[1] Ohio State Univ, Dept Ecol Evolut & Organismal Biol, Columbus, OH 43210 USA
来源：
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词：
black rat snake; DNA loci; Elaphe obsoleta; microsatellite;
D O I：
10.1046/j.1471-8286.2003.00363.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We obtained molecular markers useful for population level studies of the black rat snake (Elaphe obsoleta) by screening genomic DNA libraries enriched for dinucleotide, tetranucleotide, and pentanucleotide microsatellite repeats. Following sequencing of the positive clones, 11 pairs of primers were designed for polymorphic loci and their variability assessed in > 350 individuals from four populations in North America. The loci had between 9 and 40 alleles and observed heterozygosities ranged from 0.071 to 0.87. Some of these pairs of primers also successfully amplified DNA from two other snake species.
引用
收藏
页码：98 / 99
页数：2
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