Chronic use of marijuana decreases cannabinoid receptor binding and mRNA expression in the human brain

被引:96
作者
Villares, J. [1 ]
机构
[1] Univ Fed Sao Paulo, Dept Psychobiol, Aging & Neurodegenerat Dis Brain Bank Lab, BR-04023062 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Cannabis sativa; CB1 receptor binding; chronic drug exposure; postmortem human brain; mRNA CB1 expression; psychostimulants;
D O I
10.1016/j.neuroscience.2006.11.012
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Chronic exposure to Cannabis sativa (marijuana) produced a significant down-regulation of cannabinoid receptor in the postmortem human brain. The significant decrease in maximal binding capacity was not accompanied by changes in the affinity constant. [H-3]SR141716A binding was reduced in the caudate nucleus, putamen and in the accumbens nucleus. A significant decrease of binding sites was seen in the globus pallidus. Also in the ventral tegmental area and substantia nigra pars reticulata quantitative analysis of the density of receptors shows a significant reduction in [H-3]SR141716A binding. In Cannabis sativa user brains, compared with normal brains [H-3]SR141716A binding was reduced only in the hippocampus. The density of cannabinoid receptor 1 mRNA-positive neurons was significantly lower in Cannabis sativa users than in control brains for the caudate nucleus, putamen, accumbens nucleus and hippocampal region (CA1-CA4, areas of Ammon's horn). No hybridization was seen in the mesencephalon and globus pallidus. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:323 / 334
页数:12
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