Butyric acid production with Clostridium tyrobutyricum immobilised to PVA gel

被引:8
作者
Rebros, Martin [1 ]
Dolejs, Igor [1 ]
Stloukal, Radek [2 ]
Rosenberg, Michal [1 ]
机构
[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Biotechnol, Radlinskeho 9, Bratislava 81237, Slovakia
[2] LentiKats As, Vinici 83, Straz Pod Ralskem 47127, Czech Republic
关键词
Clostridium tyrobutyricum; Butyric acid; Immobilisation; LentiKat (R); FIBROUS-BED BIOREACTOR; ZYMOMONAS-MOBILIS; FERMENTATION; HYDROLYSATE; ENTRAPMENT; GLUCOSE; XYLOSE;
D O I
10.1016/j.procbio.2016.03.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Clostridium tyrobutyricurn was entrapped into lens-shaped polyvinyl alcohol particles (LentiKats (R)). Immobilised cells were propagated in series of repeated batches and reached average productivity of 1.74 g/L h and yield of 0.44 g/g during 31 repetitions with glucose as an initial substrate, which was 5 times higher productivity compared with a free cell system under the same conditions. Immobilised cells were successfully applied for more than 71 repeated batches and demonstrated remarkable process stability. Application of immobilised cells to a continuous system resulted in 5.8 times higher productivity compared with free cells, with 6.8 g/L of butyric acid and residual glucose of 5.8 g/L. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:704 / 708
页数:5
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