Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

被引：12

作者：

Labouesse, Celine ^{[1
,2
]}

Gabella, Chiara ^{[1
]}

Meister, Jean-Jacques ^{[1
]}

Vianay, Benoit ^{[1
,3
,4
]}

Verkhovsky, Alexander B. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Cell Biophys, Lausanne, Switzerland

[2] Univ Cambridge, MRC, Stem Cell Inst, Wellcome Trust, Cambridge CB2 1TN, England

[3] UJF, CEA, CNRS, INRA,Inst Rech Technol & Sci Vivant,UMR5168, Grenoble, France

[4] Univ Paris Diderot, Hop St Louis, AP HP, Inst Univ Hematol,INSERM,U1160, F-75010 Paris, France

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

瑞士国家科学基金会;

关键词：

CELL-SHAPE; TENSILE PROPERTIES; DYNAMICS; ISOFORM; BUNDLE;

D O I：

10.1038/srep23722

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.

引用

页数：9

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