Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins

被引：37

作者：

Dziga, Dariusz ^{[1
]}

Zielinska, Gabriela ^{[2
]}

Wladyka, Benedykt ^{[2
]}

Bochenska, Oliwia ^{[2
]}

Maksylewicz, Anna ^{[1
]}

Strzalka, Wojciech ^{[3
]}

Meriluoto, Jussi ^{[4
]}

机构：

[1] Jagiellonian Univ, Dept Plant Physiol & Dev, Fac Biochem Biophys & Biotechnol, Gronostajowa 7, PL-30387 Krakow, Poland

[2] Jagiellonian Univ, Dept Analyt Biochem, Fac Biochem Biophys & Biotechnol, Gronostajowa 7, PL-30387 Krakow, Poland

[3] Jagiellonian Univ, Dept Plant Biotechnol, Fac Biochem Biophys & Biotechnol, Gronostajowa 7, PL-30387 Krakow, Poland

[4] Abo Akad Univ, Dept Biochem, Fac Sci & Engn, Tykistokatu 6 A, Turku 20520, Finland

来源：

TOXINS | 2016年 / 8卷 / 03期

关键词：

recombinant enzymes; microcystin; biodegradation; biochemical pathway; MICROBIAL-DEGRADATION; LR; BIODEGRADATION; PATHWAY;

D O I：

10.3390/toxins8030076

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Bacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A new degradation intermediate, a hexapeptide derived from linearized microcystins by MlrC, was discovered. Furthermore, the involvement of MlrA and MlrB in further degradation of the hexapeptides was confirmed and a corrected biochemical pathway of microcystin biodegradation has been proposed.

引用

页数：13

共 24 条

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