CLONING AND EXPRESSION ANALYSIS OF KFNCED1 FROM KALIDIUM FOLIATUM (CHENOPODIACEAE) IN RESPONSE TO NACL TREATMENT

Kalidium foliatum exhibits strong tolerance to salinity. NCED1 from K foliatum was cloned, and its expression level was analysed under salt treatment. Whole plants were subjected to 0, 150, 200, 250, 300, 350 and 400 mM NaCl for 72 h. Total RNA was extracted from the leaves of 4-month-old K. foliatum seedlings. The full-length KfNCED(1) cDNA was cloned with reverse transcription PCR, the KfNCED(1) expression levels were analysed with semiquantitative RT-PCR, and the ABA contents from K. foliatum were examined. The KfNCED(1) cDNA was 1500 bp long and included an open reading frame that encoded a polypeptide of 499 amino acids. The deduced amino acid sequence of KfNCED(1) had significant similarity with that of AiNCED1. Semiquantitative RT-PCR showed that the expression of KfNCED(1) could be markedly induced by salt treatment.