Polyethylene glycol-potassium phosphate aqueous two-phase systems for countercurrent chromatography of proteins

被引：11
|
作者：
Shibusawa, Y
Yamaguchi, M
Ito, Y
机构：
[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Tokyo 19203, Japan
[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1998年 / 21卷 / 1-2期
关键词：
D O I：
10.1080/10826079808001941
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000-potassium phosphate were used for the countercurrent chromatographic separation of proteins using the type XL cross-axis coil planet centrifuge. It was found that the peak resolution of proteins is highly dependant on the pH of the solvent system. The best separation of cytochrome c, myoglobin and human serum albumin was achieved with 16.0% PEG 1000-12.5% potassium phosphate buffer at pH 9.2. The resolution and separation time were improved by pH-peak-focusing countercurrent chromatography.
引用
收藏
页码：121 / 133
页数：13
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